CAJ | 학술논문

采用微波结合链霉素抗性筛选法选育放线菌素 D 的高产菌株。通过考察链霉素对 Streptomyces rubiginosohelvolus FIM－N31菌株孢子生长情况的影响确定链霉素致死浓度，出发菌株FIM－N31的孢子经微波辐照处理后，涂布在含链霉素致死浓度（50μg／mL）的培养基平板上培养，获得了大量的链霉素抗性基因突变株。摇瓶发酵筛选突变株，结果获得一株遗传性状稳定的放线菌素D高产菌Str186，其产放线菌素D的能力比出发菌株提高了8倍以上。
채용미파결합련매소항성사선법선육방선균소 D 적고산균주。통과고찰련매소대 Streptomyces rubiginosohelvolus FIM－N31균주포자생장정황적영향학정련매소치사농도，출발균주FIM－N31적포자경미파복조처리후，도포재함련매소치사농도（50μg／mL）적배양기평판상배양，획득료대량적련매소항성기인돌변주。요병발효사선돌변주，결과획득일주유전성상은정적방선균소D고산균Str186，기산방선균소D적능력비출발균주제고료8배이상。
Microwave mutagenesis and streptomycin resistance screening was applied to screen actinomycin D high-producting strain.After testing the resistance of Streptomyce rubiginosohelvolus FIM-N31 to streptomycin, the lethal concentration of streptomycin was determined.A lot of streptomycin-resistant mutants were screened after the spores regenerated on the lethal streptomycin concentration ( 50μg/mL) media when they were treated with microwave irradiation.It was eventually found that the high-yield strain Str186 from these mutants which was stably inheritable, and the productivity was beyond eight times higher than the original strains in the rotation-flask experiments.