生物加工过程
生物加工過程
생물가공과정
Chinese Journal of Bioprocess Engineering
2015年
5期
61-66
,共6页
沈江涛%段学辉%郑希帆%张倩%牛书操
瀋江濤%段學輝%鄭希帆%張倩%牛書操
침강도%단학휘%정희범%장천%우서조
纤维素酶%混合菌发酵%预处理%SPORL%酶解糖化
纖維素酶%混閤菌髮酵%預處理%SPORL%酶解糖化
섬유소매%혼합균발효%예처리%SPORL%매해당화
cellulase%mixed fermentation%pretreatment%SPORL%enzymatic saccharification
采用稀酸、稀碱、高温稀碱、亚硫酸盐法（ SPORL法）和稀酸亚硫酸盐法（稀酸SPORL法）对粉碎稻草秸秆预处理，考察不同预处理方法对稻草基质多菌发酵产纤维素酶的影响，分析预处理前后稻草基质主要成分的变化，酶水解液中糖组分的含量。结果表明：稀酸SPORL法处理的稻草粉在固态发酵产酶和酶解糖化都具有较好的效果，所得羧甲基纤维素酶（ CMCase酶）和β葡萄糖苷酶（β G）比酶活分别达到21511?22和51508?41 U／g，同时酶水解率达到84?99％。除SPORL法外，其他预处理方式所得酶活均出现了不同程度的下降。稀酸预处理对稻草基质中的半纤维素去除效果较好，含量由20?77％下降到7?34％；稀碱高温处理对木质素脱除效果较好，Klason木质素含量由12?47％下降到7?58％。通过酶解糖化实验发现，未处理稻草粉酶水解率仅为17?82％，稀碱高温法效果最好，稻酶水解率达到91?66％。稀酸和稀酸SPORL法处理后，稻草粉基质的酶解糖化液中，戊聚糖占总糖相对含量较低，分别为7?38％和6?92％。
採用稀痠、稀堿、高溫稀堿、亞硫痠鹽法（ SPORL法）和稀痠亞硫痠鹽法（稀痠SPORL法）對粉碎稻草秸稈預處理，攷察不同預處理方法對稻草基質多菌髮酵產纖維素酶的影響，分析預處理前後稻草基質主要成分的變化，酶水解液中糖組分的含量。結果錶明：稀痠SPORL法處理的稻草粉在固態髮酵產酶和酶解糖化都具有較好的效果，所得羧甲基纖維素酶（ CMCase酶）和β葡萄糖苷酶（β G）比酶活分彆達到21511?22和51508?41 U／g，同時酶水解率達到84?99％。除SPORL法外，其他預處理方式所得酶活均齣現瞭不同程度的下降。稀痠預處理對稻草基質中的半纖維素去除效果較好，含量由20?77％下降到7?34％；稀堿高溫處理對木質素脫除效果較好，Klason木質素含量由12?47％下降到7?58％。通過酶解糖化實驗髮現，未處理稻草粉酶水解率僅為17?82％，稀堿高溫法效果最好，稻酶水解率達到91?66％。稀痠和稀痠SPORL法處理後，稻草粉基質的酶解糖化液中，戊聚糖佔總糖相對含量較低，分彆為7?38％和6?92％。
채용희산、희감、고온희감、아류산염법（ SPORL법）화희산아류산염법（희산SPORL법）대분쇄도초갈간예처리，고찰불동예처리방법대도초기질다균발효산섬유소매적영향，분석예처리전후도초기질주요성분적변화，매수해액중당조분적함량。결과표명：희산SPORL법처리적도초분재고태발효산매화매해당화도구유교호적효과，소득최갑기섬유소매（ CMCase매）화β포도당감매（β G）비매활분별체도21511?22화51508?41 U／g，동시매수해솔체도84?99％。제SPORL법외，기타예처리방식소득매활균출현료불동정도적하강。희산예처리대도초기질중적반섬유소거제효과교호，함량유20?77％하강도7?34％；희감고온처리대목질소탈제효과교호，Klason목질소함량유12?47％하강도7?58％。통과매해당화실험발현，미처리도초분매수해솔부위17?82％，희감고온법효과최호，도매수해솔체도91?66％。희산화희산SPORL법처리후，도초분기질적매해당화액중，무취당점총당상대함량교저，분별위7?38％화6?92％。
Straw powder was pretreated by diluted acid, diluted alkali, high temperature with diluted alkali,sulfite pretreatment(SPORL),sulfite pretreatment with diluted acid(SPORL with diluted acid). The effects of pretreatment on the cellulases production by solid state fermentation and the yield of fermentable sugars hydrolyzed were studied. Results showed when the straw substrate pretreated by diluted acid with SPORL for cellulase fermentation,carboxymethyl cellulase( CMCase) and β?glucosidase(β?G) activities reached 21 511?22 U/g and 51 508?41 U/g, respectively. The hydrolyze rate of cellulases reached 84?99%. The cellulase activities decreased in different degrees excluding SPORL. The diluted acid method was better to remove hemicelluloses, and the content of hemicelluloses decreased from 20?77% to 7?34% in pretreated straw substrate. The high temperature with dilute alkali had a significant effect on klason lignin removal. The content of klason lignin decreased from 12?47% to 7?58% in straw substrate.Further cellulose enzymolysis experiment showed that the enzymatic hydrolysis conversion rate of untreated straw powder was only 17?82%,while the high temperature with dilute alkali pretreatment was the best method,the hydrolyze rate of pretreated straw powder could reach 91?66%. In the enzymolysis liquid of straw substrate pretreated by dilute acid or SPORL with dilute acid,the content of pentosan was relative low,only 7?38% and 6?92%,respectively.
