CAJ | 학술논문

褪黑素（melatonin）是松果腺分泌的一种神经内分泌激素，主要通过其膜受体（MR）的介导参与调控动物生理节律、季节性繁殖和卵巢功能等生理过程。melatonin 的高亲和性膜受体分为 MT1和 MT2两种亚型，本试验采用 RT-qPCR 和 Western blot 方法研究了 MT1和 MT2在妊娠期奶山羊卵巢中的表达特点。结果表明，MT1和 MT2在妊娠不同阶段奶山羊卵巢中都有表达，并表现为相同的表达模式，妊娠中期表达水平最高，妊娠前期和妊娠中期表达水平极显著高于妊娠后期（P ＜0．01）。MT1和 MT2在不同妊娠期奶山羊卵巢中的表达特点与黄体的功能一致，提示褪黑素可能通过 MT1和 MT2的介导对妊娠期奶山羊黄体功能发挥调节作用。
퇴흑소（melatonin）시송과선분비적일충신경내분비격소，주요통과기막수체（MR）적개도삼여조공동물생리절률、계절성번식화란소공능등생리과정。melatonin 적고친화성막수체분위 MT1화 MT2량충아형，본시험채용 RT-qPCR 화 Western blot 방법연구료 MT1화 MT2재임신기내산양란소중적표체특점。결과표명，MT1화 MT2재임신불동계단내산양란소중도유표체，병표현위상동적표체모식，임신중기표체수평최고，임신전기화임신중기표체수평겁현저고우임신후기（P ＜0．01）。MT1화 MT2재불동임신기내산양란소중적표체특점여황체적공능일치，제시퇴흑소가능통과 MT1화 MT2적개도대임신기내산양황체공능발휘조절작용。
Melatonin is a kind of neuroendocrinologic hormone secreted by the pineal gland.Studies showed that melatonin has involved in the regulation of the complex physiological processes like circadian rhythms, seasonal breeding and ovarian function through the membrane receptors.The high affinity melatonin mem-brane receptors were described as two subtypes:MT1 and MT2.The objective of this study was to evalu-ate the expressions of MT1 and MT in the ovaries of dairy goats during pregnancy using real-time RT-PCR and Western blot assays.Results showed that similar patterns of MT1 and MT2 expressions were ob-served,the abundance of transcripts and proteins of MT1 and MT2 were significantly greater in earlier and mid-stages than in later-stage (P <0.01).The profiles of MT1 and MT2 expression in the ovaries were roughly coincident with the functional activity of corpora luteum and paralleled the pattern of progesterone secretion,which is suggestive of a potential functional role of melatonin in the regulation of luteal function of dairy goats during pregnancy through MT1 and MT2.