CAJ | 학술논문

目的探讨白细胞介素17(IL-17)及其受体调控酪氨酸激酶(JAK)/转录激活因子-3(STAT3)信号通路促进喉癌血管生成的作用. 方法收集喉癌手术切除标本70 例,癌组织通过不同病理分级,采取70 例喉癌患者的外周血. 采用ELISA 法检测外周血中 IL-17、血管内皮生长因子 A ( VEGF-A)的浓度,分析其与临床病理特征的关系. Western blot 法检测喉癌组织中IL-17、IL-17受体( IL-17R)、JAK1/2、p-JAK1/2、STAT3、p-STAT3、VEGF-A、环氧合酶-2 ( COX-2 )、基质金属蛋白酶-9(MMP-9)等信号分子水平的变化. 结果喉癌患者血清中IL-17、VEGF-A的含量与喉癌的分化程度、淋巴结有无转移和TNM分期有关( P<0. 05 ) , Western blot结果显示在喉癌发展的不同时期,随着喉癌的恶化, IL-17、IL-17R的表达逐渐增强,相关信号通路分子JAK1/2、p-JAK1/2、STAT3、p-STAT3、VEGF-A、COX-2、MMP-9 表达也显著增加. 结论 IL-17可能调控JAK/STAT3信号通路,在喉癌的血管生成中起到重要的作用.
목적 탐토백세포개소17(IL-17)급기수체조공락안산격매(JAK)/전록격활인자-3(STAT3)신호통로촉진후암혈관생성적작용. 방법 수집후암수술절제표본70 례,암조직통과불동병리분급,채취70 례후암환자적외주혈. 채용ELISA 법검측외주혈중 IL-17、혈관내피생장인자 A ( VEGF-A)적농도,분석기여림상병리특정적관계. Western blot 법검측후암조직중IL-17、IL-17수체( IL-17R)、JAK1/2、p-JAK1/2、STAT3、p-STAT3、VEGF-A、배양합매-2 ( COX-2 )、기질금속단백매-9(MMP-9)등신호분자수평적변화. 결과후암환자혈청중IL-17、VEGF-A적함량여후암적분화정도、림파결유무전이화TNM분기유관( P<0. 05 ) , Western blot결과현시재후암발전적불동시기,수착후암적악화, IL-17、IL-17R적표체축점증강,상관신호통로분자JAK1/2、p-JAK1/2、STAT3、p-STAT3、VEGF-A、COX-2、MMP-9 표체야현저증가. 결론 IL-17가능조공JAK/STAT3신호통로,재후암적혈관생성중기도중요적작용.
Objective To investigate IL-17 and its receptor regulate JAK/STAT3 signaling pathway in the angio-genesis of laryngeal cancer. Methods Serum levels of IL-17 and VEGF-A were determined in 70 laryngeal carci-noma patients using ELISA. The levels as well as clinicopathological features were analyzed. Western blot assay was used to detect the expressions of IL-17,IL-17R,JAK1/2,p-JAK1/2,STAT3,p-STAT3,VEGF-A,COX-2,MMP-9 in 70 cases of laryngeal carcinoma. Statistics methods were applied to analyze the results. Results Serum levels of both IL-17 and VEGF-A levels in patients were related to the degree of differentiation of laryngeal cancer, lymph node metastasis and TNM stage (P<0. 05), were unrelated to age,sex,primary tumor site. Western blot analysis showed that at different times in development of laryngeal cancer, with the deterioration of laryngeal cancer, IL-17, IL-17R expression gradually increased, JAK1/2,p-JAK1/2,STAT3,p-STAT3,VEGF-A,COX-2 and MMP-9 ex-pressions were also significantly increased. Conclusion IL-17 may play an important role in angiogenesis of laryn-geal cancer via regulating JAK/STAT3 signaling pathway.