CAJ | 학술논문

脑苷脂A和B具有显著的镇痛作用和脑神经保护作用。鸡枞菌（ Termitomyces clypeatus）子实体含有脑苷脂A和B，但是野生鸡枞菌子实体资源稀少。笔者在前期研究中以野生鸡枞菌子实体为原料，通过组织分离、菌种纯化及筛选，培育出菌株CTM 1。本研究中，采用分子生物学方法鉴定了菌株CTM 1与鸡枞菌的菌种同源性，测定了该菌株平板培养、液体培养中菌丝体的生长速率和脑苷脂含量。实验结果表明：菌株CTM 1及其出发野生子实体与Termitomyces clypeatus的同源性为99％。在PDAY平板培养中，菌落直径的增长速率在27 d内基本稳定在1?5 mm／d。比菌落直径增长速率在1 d时为0?21 d－1，然后逐渐下降。在DP t液体培养中，9 d以后为菌丝体生长的稳定期，此时的生物量为3?57 g／L。比菌丝体生长速率在1 d时为2?00 d－1，然后逐渐下降。菌丝体的脑苷脂A、B含量分别为0?152％、0?066％。由此可见，鸡枞菌CTM 1的大规模培养可以成为脑苷脂A、B原料生产的有效途径。
뇌감지A화B구유현저적진통작용화뇌신경보호작용。계종균（ Termitomyces clypeatus）자실체함유뇌감지A화B，단시야생계종균자실체자원희소。필자재전기연구중이야생계종균자실체위원료，통과조직분리、균충순화급사선，배육출균주CTM 1。본연구중，채용분자생물학방법감정료균주CTM 1여계종균적균충동원성，측정료해균주평판배양、액체배양중균사체적생장속솔화뇌감지함량。실험결과표명：균주CTM 1급기출발야생자실체여Termitomyces clypeatus적동원성위99％。재PDAY평판배양중，균락직경적증장속솔재27 d내기본은정재1?5 mm／d。비균락직경증장속솔재1 d시위0?21 d－1，연후축점하강。재DP t액체배양중，9 d이후위균사체생장적은정기，차시적생물량위3?57 g／L。비균사체생장속솔재1 d시위2?00 d－1，연후축점하강。균사체적뇌감지A、B함량분별위0?152％、0?066％。유차가견，계종균CTM 1적대규모배양가이성위뇌감지A、B원료생산적유효도경。
Cerebrosides A and B have significant effects of analgesia and brain protection. Fruiting bodies of Termitomyces contains cerebrosides A and B, but wild Termitomyces resources are scarce. In author′s previous work, a strain CTM?1 was developed by means of tissue isolation, strain purification and screening,using the fresh fruiting bodies of wild Termitomyces as raw material. In the present study, the sequence homology of CTM?1 and Termitomyces was identified with a molecular?biological method. The mycelium growth rate in plate and liquid culture,and the cerebroside content in mycelia were investigated for the strain CTM?1. It was shown that the stain CTM?1 and its parental fruiting bodies had a sequence homology up to 99% with Termitomyces clypeatus. In PDAY plate culture,the colony?diameter increasing rate was constant at 1?5 mm/d in the period between and 27 d. The specific colony?diameter increasing rate was 0?21 d-1 at 1 d,and decreased gradually after then.In DPt liquid culture,the growth of mycelia entered the stable phase at 9 d,and the biomass was 3?57 g/L. The specific mycelium growth rate was 2?00 d-1 at 1 d,and decreased gradually after then. The content of cerebrosides A and B in mycelia was 0?152% and 0?066%, respectively. Consequently, the large?scale culture of T. clypeatus CTM?1 can be considered as an effective way for the production of raw material containing cerebrosides A and B.