CAJ | 학술논문

bHLH类转录因子广泛存在于植物中，在花的形态建成、光和激素信号转导等方面发挥着重要作用，但其表达调控机理尚待深入研究。从玉米中克隆了bHLH类转录因子ABP7，初步研究结果显示它可能参与了籽粒形成、激素应答等多个信号转导途径的调控。为进一步阐明ABP7基因上游调控途径，克隆了ABP7的5′侧翼序列，利用原生质体瞬时转化体系验证了其启动子活性，并对其顺式作用元件进行了分析。结果显示，位于ABP7基因起始密码子上游长为2266 bp的DNA序列具有启动子活性，能够启动报告基因的表达，并且存在许多潜在的与GA、IAA和ABA激素信号转导和逆境胁迫响应相关的元件，为进一步鉴定ABP7基因发挥作用的信号途径、上游调控因子和分子机理以及ABP7基因在玉米生长发育和逆境应答中的功能奠定了基础。
bHLH류전록인자엄범존재우식물중，재화적형태건성、광화격소신호전도등방면발휘착중요작용，단기표체조공궤리상대심입연구。종옥미중극륭료bHLH류전록인자ABP7，초보연구결과현시타가능삼여료자립형성、격소응답등다개신호전도도경적조공。위진일보천명ABP7기인상유조공도경，극륭료ABP7적5′측익서렬，이용원생질체순시전화체계험증료기계동자활성，병대기순식작용원건진행료분석。결과현시，위우ABP7기인기시밀마자상유장위2266 bp적DNA서렬구유계동자활성，능구계동보고기인적표체，병차존재허다잠재적여GA、IAA화ABA격소신호전도화역경협박향응상관적원건，위진일보감정ABP7기인발휘작용적신호도경、상유조공인자화분자궤리이급ABP7기인재옥미생장발육화역경응답중적공능전정료기출。
Basichelix-loop-helix ( bHLH) transcription factors are widely present in plants, which play important roles in flower morphogenesis, light signal transduction and hormonal signal transduction.However, the molecular mechanisms regulating their expression remain largely unknown.In this paper, we previously cloned a bHLH transcription factor gene from maize, designated ABP7, and preliminary data suggestted that it might be involved in multiple signal transduction pathways such as kernel development and hormone response.For further elucidation of the upstream pathways regulating ABP7 expression, we cloned the 5′flanking sequence of ABP7, verified its promoter activity by transient expression in maize protoplasts, and predicted its cis-acting elements.The results showed that the cloned 2 266 bp 5′flanking region is a functional promoter which is capable to drive the expression of downstream reporter, and contains many potential cis-acting elements involved in the signaling of hormones such as GA、IAA and ABA, and responses to adverse environments.These results set a base for future studies in identification of upstream regulatory networks that control ABP7 expression, and in unraveling the specific roles of ABP7 in plant growth, development and stress response.