CAJ | 학술논문

目的：建立并评价一种简单快速的逆转录环-介导的等温扩增(直接RT-LAMP)检测人肠道病毒71(EV71)的实验方法。方法无需RNA提取，用热处理标本后用直接RT-LAMP方法检测EV71，并用临床收集的290份咽试标本评价其灵敏度和特异性。结果直接RT-LAMP和RT-LAMP实验完全符合率为92.4%(268/290)，直接RT-LAMP和qRT-PCR完全符合率为88.9%。在RT-LAMP或直接RT-LAMP实验中没有发现假阳性。与RT-LAMP比较，直接RT-LAMP的灵敏度和特异性分别为90.3%和100%；与qRT-PCR比较，其敏感性和特异性分别为86.8%和100%。结论直接RT-LAMP方法能被开发成简单快速检测EV71和其他病原体的方法。
목적：건립병평개일충간단쾌속적역전록배-개도적등온확증(직접RT-LAMP)검측인장도병독71(EV71)적실험방법。방법무수RNA제취，용열처리표본후용직접RT-LAMP방법검측EV71，병용림상수집적290빈인시표본평개기령민도화특이성。결과직접RT-LAMP화RT-LAMP실험완전부합솔위92.4%(268/290)，직접RT-LAMP화qRT-PCR완전부합솔위88.9%。재RT-LAMP혹직접RT-LAMP실험중몰유발현가양성。여RT-LAMP비교，직접RT-LAMP적령민도화특이성분별위90.3%화100%；여qRT-PCR비교，기민감성화특이성분별위86.8%화100%。결론직접RT-LAMP방법능피개발성간단쾌속검측EV71화기타병원체적방법。
Objective To develop a simple and rapid direct reverse transcription loop-mediated isothermal am-plification (direct RT-LAMP) experimental method for detecting enterovirus 71 (EV71) infection, and the sensitivity and specificity of this method using clinical nasopharyngeal swab specimens for the detection of EV71 were evaluated. Methods Direct RT-LAMP without RNA extraction and with heat-treatment was used to detect EV71 infection in 290 nasopharyngeal swab specimens. The sensitivity and specificity were evaluated. Results The accordance rate of direct RT-LAMP and RT-LAMP was 92.4%(268/290), and that of direct RT-LAMP and qRT-PCR was 88.9%. No false posi-tive was found in direct RT-LAMP or RT-LAMP. The clinical performance demonstrated the sensitivity and specificity of direct RT-LAMP was 90.3%and 100%compared to RT-LAMP, and 86.8%and 100%compared to qRT-PCR, re-spectively. Conclusion Direct RT-LAMP method can potentially be developed for simple and rapid screening of EV71 and other pathogens.