海南医学
海南醫學
해남의학
Hainan Medical Journal
2015年
18期
2723-2724
,共2页
林丽华%钟娜%乔凤%张丽芬
林麗華%鐘娜%喬鳳%張麗芬
림려화%종나%교봉%장려분
人乳头瘤病毒%FQ-PCR%感染率%尖锐湿疣
人乳頭瘤病毒%FQ-PCR%感染率%尖銳濕疣
인유두류병독%FQ-PCR%감염솔%첨예습우
Human papillomavivus%Fluorescent quantitation PCR%Infection rate%Condyloma acuminata
目的：了解海口地区人乳头瘤病毒(HPV)感染的情况，为HPV感染患者的治疗及监测提供科学依据。方法采用实时荧光定量PCR方法(FQ-PCR)对海口地区615例性病门诊就诊者泌尿系分泌物标本的HPV6，11型和HR-HPV (8个型)进行检测。结果615例样本中检出HPV阳性者302例，感染率为49.11%(302/615)，其中HPV6，11-DNA阳性130例，阳性率为21.14%(130/615)，HR-HPV-DNA阳性126例，阳性率为20.49%(126/615)，HPV6，11型合并HR-HPV阳性46例，占7.48%(46/615)。HPV6，11低危型与HR-HPV混合高危型比较差异无统计学意义(P>0.05)。302例HPV感染者中尖锐湿疣(CA)患者187例，占61.92%(187/302)，检出HPV6,11-DNA阳性126例，占67.38%(126/187)，HR-HPV-DNA阳性19例，占10.16%(19/187)，HPV6,11型和HR-HPV混合感染42例，占22.46%(42/187)。CA患者感染以HPV6，11低危型为主，HPV6，11/HR-HPV次之，少部分为高危混合型HR-HPV感染，各组比较差异有统计学意义(P<0.01)。结论尖锐湿疣主要的基因型感染是低危型HPV6,11，高危型HPV感染多以混合感染形式存在。荧光定量PCR方法检测尖锐湿疣患者HPV基因型具有快速、准确的优点，是临床HPV感染分型检测的有效方法。
目的：瞭解海口地區人乳頭瘤病毒(HPV)感染的情況，為HPV感染患者的治療及鑑測提供科學依據。方法採用實時熒光定量PCR方法(FQ-PCR)對海口地區615例性病門診就診者泌尿繫分泌物標本的HPV6，11型和HR-HPV (8箇型)進行檢測。結果615例樣本中檢齣HPV暘性者302例，感染率為49.11%(302/615)，其中HPV6，11-DNA暘性130例，暘性率為21.14%(130/615)，HR-HPV-DNA暘性126例，暘性率為20.49%(126/615)，HPV6，11型閤併HR-HPV暘性46例，佔7.48%(46/615)。HPV6，11低危型與HR-HPV混閤高危型比較差異無統計學意義(P>0.05)。302例HPV感染者中尖銳濕疣(CA)患者187例，佔61.92%(187/302)，檢齣HPV6,11-DNA暘性126例，佔67.38%(126/187)，HR-HPV-DNA暘性19例，佔10.16%(19/187)，HPV6,11型和HR-HPV混閤感染42例，佔22.46%(42/187)。CA患者感染以HPV6，11低危型為主，HPV6，11/HR-HPV次之，少部分為高危混閤型HR-HPV感染，各組比較差異有統計學意義(P<0.01)。結論尖銳濕疣主要的基因型感染是低危型HPV6,11，高危型HPV感染多以混閤感染形式存在。熒光定量PCR方法檢測尖銳濕疣患者HPV基因型具有快速、準確的優點，是臨床HPV感染分型檢測的有效方法。
목적：료해해구지구인유두류병독(HPV)감염적정황，위HPV감염환자적치료급감측제공과학의거。방법채용실시형광정량PCR방법(FQ-PCR)대해구지구615례성병문진취진자비뇨계분비물표본적HPV6，11형화HR-HPV (8개형)진행검측。결과615례양본중검출HPV양성자302례，감염솔위49.11%(302/615)，기중HPV6，11-DNA양성130례，양성솔위21.14%(130/615)，HR-HPV-DNA양성126례，양성솔위20.49%(126/615)，HPV6，11형합병HR-HPV양성46례，점7.48%(46/615)。HPV6，11저위형여HR-HPV혼합고위형비교차이무통계학의의(P>0.05)。302례HPV감염자중첨예습우(CA)환자187례，점61.92%(187/302)，검출HPV6,11-DNA양성126례，점67.38%(126/187)，HR-HPV-DNA양성19례，점10.16%(19/187)，HPV6,11형화HR-HPV혼합감염42례，점22.46%(42/187)。CA환자감염이HPV6，11저위형위주，HPV6，11/HR-HPV차지，소부분위고위혼합형HR-HPV감염，각조비교차이유통계학의의(P<0.01)。결론첨예습우주요적기인형감염시저위형HPV6,11，고위형HPV감염다이혼합감염형식존재。형광정량PCR방법검측첨예습우환자HPV기인형구유쾌속、준학적우점，시림상HPV감염분형검측적유효방법。
Objective To explore human papillomavirus (HPV) infection in people in Haikou, China, and provide a basis for the treatment and monitoring of HPV-infected patients. Methods The urinary secretion speci-mens of 615 patients from Out-patient Department of Venereal Diseases were analyzed with real-time fluorescent quantitation PCR (FQ-PCR) for HPV6,11 and HR-HPV (8 types). Results Of the 615 specimens, 302 (49.11%, 302/615) were positive of HPV, including 130 (21.14%, 130/302) positive of HPV6,11 DNA, 126 (20.49%, 126/302) posi-tive of HR-HPV DNA, and 46 (7.48%, 46/302) positive of both HPV6,11 and HR-HPV DNA. There was no statistical-ly significant difference between the low-risk group (HPV6,11) and the high-risk group (HPV6,11 combined with HR-HPV), P>0.05. Of the 302 HPV-infected patients, 187 (61.92%, 187/302) had condyloma acuminata (CA), out of which 126 (67.38%, 126/187) were positive for HPV6,11 DNA, 19 (10.16%, 19/187) positive for HR-HPV DNA and 42 (22.46%, 42/187) positive for both HPV6,11 and HR-HPV DNA. Infection with low-risk HPV6,11 was mostly seen in these CA patients, followed by mixed infection with both HPV6,11 and HR-HPV and then by high-risk infec-tion with HR-HPV, where the differences among groups were significant (P<0.01). Conclusion The main genotype of CA is low-risk HPV6,11, while the high-risk genotype of HPV often exists in a mixed status. Being rapid and pre-cise in the determination of HPV genotype in CA patients, fluorescent quantitation PCR is an effective clinical method for classifying HPV infection.
