CAJ | 학술논문

目的：了解荧光定量PCR检测解脲脲原体（Uu）敏感性、特异性及其临床应用价值。方法收集海口人民市医院2013年3~5月妇产科门诊泌尿生殖道感染患者生殖道标本共154例，同时进行荧光定量PCR和液-固体培养法检测Uu，以液-固体培养法作为金标准，对荧光定量PCR诊断试验进行方法学评价。结果154例标本中，液-固体培养法即金标准检测阳性64例，荧光定量PCR检测阳性69例，两者同时阳性63例，同时阴性84例，荧光定量PCR检测Uu敏感性是98.44%(63/64)，特异性是93.33%(84/90)，假阳性率是6.67%(6/84)，假阴性率是1.56%(1/64)，阳性预测值是91.30%(63/69)，阴性预测值是98.82%(84/85)，总符合率是95.45%(147/154)。χ2检验显示，两种方法检测结果差异无统计学意义(P>0.05)，计算Kappa>0.75，表明两种方法检测结果的一致性程度较好。结论荧光定量PCR方法具有灵敏度高、特异好、检测快速的优点，且与培养法一致性也较高，具有一定的临床应用价值。
목적：료해형광정량PCR검측해뇨뇨원체（Uu）민감성、특이성급기림상응용개치。방법수집해구인민시의원2013년3~5월부산과문진비뇨생식도감염환자생식도표본공154례，동시진행형광정량PCR화액-고체배양법검측Uu，이액-고체배양법작위금표준，대형광정량PCR진단시험진행방법학평개。결과154례표본중，액-고체배양법즉금표준검측양성64례，형광정량PCR검측양성69례，량자동시양성63례，동시음성84례，형광정량PCR검측Uu민감성시98.44%(63/64)，특이성시93.33%(84/90)，가양성솔시6.67%(6/84)，가음성솔시1.56%(1/64)，양성예측치시91.30%(63/69)，음성예측치시98.82%(84/85)，총부합솔시95.45%(147/154)。χ2검험현시，량충방법검측결과차이무통계학의의(P>0.05)，계산Kappa>0.75，표명량충방법검측결과적일치성정도교호。결론형광정량PCR방법구유령민도고、특이호、검측쾌속적우점，차여배양법일치성야교고，구유일정적림상응용개치。
Objective To evaluate the sensitivity, specificity, and application of fluorescence quantitative PCR (FQ-PCR) for detection of Ureaplasma urealyticum (Uu) in female genital tract. Methods A total of 154 speci-mens of patients with genital tract infections were detected by FQ-PCR and liquid-solid culture at the same time. All specimens were collected in Haikou People's Hospital from March 2013 to May 2013. Results In the 154 specimens, 64 and 69 samples were revealed as positive according to the golden standard method and FQ-PCR method respective-ly, with 63 samples positive and 84 negative for both the golden standard method and FQ-PCR method. For FQ-PCR, the sensitivity rate was 98.44%(63/64), and the specificity rate was 93.33% (84/90), with the false positive rate of 6.67%(6/84), the false negative rate of 1.56%(1/64), positive predictive value of 91.30%(63/69), negative predictive value of 98.82%(84/85), and the total coincidence rate of 95.45%(147/154).χ2 test results showed that there was no statistical significant difference between the results of the two methods (P>0.05), and the consistency was good (Kap-pa>0.75). Conclusion FQ-PCR has the advantages of high sensitivity, good specificity, rapid detection, and high con-sistency with culture, and thus has certain clinical application value.