CAJ | 학술논문

为探讨复方黄芪制剂对鸡新城疫灭活疫苗免疫效果的影响，将300只1日龄海兰褐雏鸡随机分成Ⅰ组（低剂量添加组）、Ⅱ组（中剂量添加组）、Ⅲ组（高剂量添加组）、Ⅳ组（阴性对照组）和Ⅴ组（阳性对照组）。7日龄时，除阴性对照组不免疫外，其余各组鸡均颈背部皮下注射新城疫灭活疫苗，0．25 mL／羽，同时Ⅰ、Ⅱ和Ⅲ组分别按10、20、40 g／kg 体重，灌服复方黄芪制剂，每天1次，连续5 d。然后在免疫后第7、14、21、28、35天时，从各组中随机选取8只健康鸡无菌采集心血1．0 mL，分离血清，用于鸡新城疫 HI 抗体、SOD 和 IFN-γ的测定。结果表明，新城疫 HI 抗体效价，血清中 SOD 和 IFN-γ的活性或浓度，复方黄芪中剂量组均高于其余各组，且与低剂量添加组、阳性对照组相比，呈显著（P ＜0．05）或极显著差异（P ＜0．01）。说明复方黄芪按20 g／kg 体重的剂量给雏鸡口服，能显著提高雏鸡的新城疫 HI 抗体水平，同时也能提高雏鸡SOD 和 IFN-γ的水平。
위탐토복방황기제제대계신성역멸활역묘면역효과적영향，장300지1일령해란갈추계수궤분성Ⅰ조（저제량첨가조）、Ⅱ조（중제량첨가조）、Ⅲ조（고제량첨가조）、Ⅳ조（음성대조조）화Ⅴ조（양성대조조）。7일령시，제음성대조조불면역외，기여각조계균경배부피하주사신성역멸활역묘，0．25 mL／우，동시Ⅰ、Ⅱ화Ⅲ조분별안10、20、40 g／kg 체중，관복복방황기제제，매천1차，련속5 d。연후재면역후제7、14、21、28、35천시，종각조중수궤선취8지건강계무균채집심혈1．0 mL，분리혈청，용우계신성역 HI 항체、SOD 화 IFN-γ적측정。결과표명，신성역 HI 항체효개，혈청중 SOD 화 IFN-γ적활성혹농도，복방황기중제량조균고우기여각조，차여저제량첨가조、양성대조조상비，정현저（P ＜0．05）혹겁현저차이（P ＜0．01）。설명복방황기안20 g／kg 체중적제량급추계구복，능현저제고추계적신성역 HI 항체수평，동시야능제고추계SOD 화 IFN-γ적수평。
To investigate the effects of compound Astragalus preparation on the immune efficacy of the in-activated Newcastle disease vaccine in chickens,300 one day-old Hyline brown chickens were randomly di-vided into groupⅠ(low-dose group),group Ⅱ (moderate-dose group ),group Ⅲ (high-dose group ),groupⅣ (negative control group)and group Ⅴ(positive control group ).When the chickens were 7 days of age, they were subcutaneously injected with the inactivated Newcastle disease vaccine except the negative con-trol group,each of 0.25 mL.Meanwhile,the chickens in the group Ⅰ,group Ⅱ and group Ⅲ were orally gavaged with the compound Astragalus preparations as 10 g/kg,20 g/kg and 40 g/kg body weight,respec-tively,once a day for 5 consecutive days.Then at the 7 d,14 d,21 d,28 d and 35 d after immunization,eight healthy chickens were randomly selected from each group and 1.0 mL blood were collected,and sera were separated for the detection of ND-HI antibodies,SOD and IFN-γ.The results were as follows:Newcastle disease HI antibody levels,serum activity or concentration of SOD and IFN-γin the compound Astragalus moderate-dose group were higher than that of other groups,and compared with the low-dose group and the positive control group,there were significant or very significant differences.The study showed that the compound Astragalus preparations,orally gavaged with 20 g/kg body weight dose,can significantly im-prove ND-HI antibody levels and the seral activities or concentrations of SOD and IFN-γin chickens.