CAJ | 학술논문

为了研究瞬时温度的提高对培养基加工的影响，通过不同的固定温度处理培养基，考察不同温度长时间处理后的培养基对CHO细胞的生长和表达的影响。结果表明，40℃处理后的效果最佳，处理工序与对照工艺相比，大大简化，产能有很大提升，为大规模生产培养基设备的选型和生产参数设置提供理论依据，且提高了设备的生产效率。
위료연구순시온도적제고대배양기가공적영향，통과불동적고정온도처리배양기，고찰불동온도장시간처리후적배양기대CHO세포적생장화표체적영향。결과표명，40℃처리후적효과최가，처리공서여대조공예상비，대대간화，산능유흔대제승，위대규모생산배양기설비적선형화생산삼수설치제공이론의거，차제고료설비적생산효솔。
In order to study instantaneous temperature's effects on serum?free medium, following limit experiments are designed,keeping the serum?free medium under different temperature for a long time to study the effects of medium under different temperature on the growth of CHO cells . The results show that 40 ℃ was the best temperature,it also reduces the procedure of medium manufacturing. It will provide a theoretical basis for the selection of medium producing equipment and production parameters, and improves production efficiency of the equipment.