CAJ | 학술논문

目的：探讨新降糖颗粒对链脲佐菌素诱导的2型糖尿病大鼠糖代谢关键酶活性及肝功能的影响。方法：50只雄性SD大鼠除8只给予普通饲料喂养作为正常组外，其余大鼠均采用高脂饲料喂养4周，一次性腹腔注射链脲佐菌素（STZ）40 mg·kg-1建立2型糖尿病大鼠模型，随机分为模型组、二甲双胍组（0.15 g·kg-1）和新降糖颗粒高、低剂量组（12.64、6.32 g·kg-1），每天灌胃给药1次，连续8周。干预8周后，检测大鼠空腹血糖（FBG）、空腹胰岛素（FINS）、糖化血红蛋白（HbA1c）、肝糖原，检测肝功能指标丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）、γ-谷氨酰转移酶（γ-GT）及糖代谢关键酶己糖激酶（HK）、果糖-6-磷酸激酶（PFK）、丙酮酸激酶（PK）及葡萄糖-6-磷酸脱氢酶（G6PDH）活性。结果：与模型组比较，新降糖颗粒能明显降低FBG、FINS、内环境稳态模型法胰岛素抵抗指数（HOMA-IR）、HbA1c及肝功能指标ALT、AST、ALP及γ-GT（P<0.05，P<0.01），增加肝糖原含量（P<0.01），增强糖代谢关键酶HK、PFK、PK及G6PDH的活性（P<0.05，P<0.01）。结论：新降糖颗粒能有效改善2型糖尿病大鼠的症状，推测其机制可能与新降糖颗粒增强肝脏糖代谢关键酶HK、PFK、PK及G6PDH活性，促进肝糖原合成，改善肝功能，下调血清FINS水平，改善胰岛素抵抗等多因素有关，最终降低了2型糖尿病大鼠的FBG、HbA1c水平。
목적：탐토신강당과립대련뇨좌균소유도적2형당뇨병대서당대사관건매활성급간공능적영향。방법：50지웅성SD대서제8지급여보통사료위양작위정상조외，기여대서균채용고지사료위양4주，일차성복강주사련뇨좌균소（STZ）40 mg·kg-1건립2형당뇨병대서모형，수궤분위모형조、이갑쌍고조（0.15 g·kg-1）화신강당과립고、저제량조（12.64、6.32 g·kg-1），매천관위급약1차，련속8주。간예8주후，검측대서공복혈당（FBG）、공복이도소（FINS）、당화혈홍단백（HbA1c）、간당원，검측간공능지표병안산안기전이매（ALT）、천문동안산안기전이매（AST）、감성린산매（ALP）、γ-곡안선전이매（γ-GT）급당대사관건매기당격매（HK）、과당-6-린산격매（PFK）、병동산격매（PK）급포도당-6-린산탈경매（G6PDH）활성。결과：여모형조비교，신강당과립능명현강저FBG、FINS、내배경은태모형법이도소저항지수（HOMA-IR）、HbA1c급간공능지표ALT、AST、ALP급γ-GT（P<0.05，P<0.01），증가간당원함량（P<0.01），증강당대사관건매HK、PFK、PK급G6PDH적활성（P<0.05，P<0.01）。결론：신강당과립능유효개선2형당뇨병대서적증상，추측기궤제가능여신강당과립증강간장당대사관건매HK、PFK、PK급G6PDH활성，촉진간당원합성，개선간공능，하조혈청FINS수평，개선이도소저항등다인소유관，최종강저료2형당뇨병대서적FBG、HbA1c수평。
This article was aimed to investigate the effect of theXin-Jiang-Tang(XJT) Granules on activity of hepatic glycometabolic key enzymes and liver function in streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats. Fifty male SD rats were randomly divided into the normal control group with 8 rats fed with normal diet, and other rats in the model group fed with high-fat diet for 4 weeks. And then, STZ (40 mg·kg-1) was peritoneally injected once to induce T2DM rat model. The model rats were randomly divided into the T2DM model group, metformin (0.15 g·kg-1) group, and high-dose (12.64 g·kg-1) and low-dose (6.32 g·kg-1) XJT Granules group. The intragastric administration was given once a day for 8 weeks. After 8-week intervention, fasting blood glucose (FBG), fasting serum insulin (FINS), glycosylated hemoglobin (HbA1c), hepatic glycogen, serum ALT, AST, ALP,γ-GT and the activity of HK, PFK, PK, and G6PDH were detected. The results showed that comparing with the model group, XJT Granules group can obvious reduce FBG, FINS, HOME-IR, HbA1c and liver function indexes such as ALT, AST, ALP,γ-GT levels (P < 0.05,P < 0.01), increase the content of hepatic glycogen (P < 0.01), and the activity of HK, PFK, PK and G6PDH (P < 0.05,P < 0.01). It was conclude that XJT Granules can remarkably regulate glycometabolism of diabetic model rats and the regulatory mechanism may be associated with the increasing of HK, PFK, PK and G6PDH activity, promoting the synthesis of hepatic glycogen, improving liver function, downregulating FINS level, improving insulin resistance and eventually decreasing the level of FBG and HbA1c of T2DM rats.