CAJ | 학술논문

探讨 H6N6亚型禽流感病毒在鸭群中的流行状况，为贵州省流感疫情动态监测及防控提供依据。从贵州三穗县某养鸭殖场采集50份泄殖腔棉拭子，处理后经过绒毛尿囊腔接种9日龄非免疫鸡胚，通过血凝（HA）和血凝抑制试验（HI）分离到1株既不是新城疫病毒，也不是 H5、H7和 H9亚型禽流感的病毒；提取病毒 RNA，通过禽流感病毒 HA 和 NA 基因通用引物扩增得到相应的 HA 和 NA 基因片段。经过测序比对分析证实所分离病毒为 H6N6亚型禽流感病毒。贵州鸭群中存在 H6N6亚型禽流感病毒，为进一步研究其起源和致病性提供了重要依据。
탐토 H6N6아형금류감병독재압군중적류행상황，위귀주성류감역정동태감측급방공제공의거。종귀주삼수현모양압식장채집50빈설식강면식자，처리후경과융모뇨낭강접충9일령비면역계배，통과혈응（HA）화혈응억제시험（HI）분리도1주기불시신성역병독，야불시 H5、H7화 H9아형금류감적병독；제취병독 RNA，통과금류감병독 HA 화 NA 기인통용인물확증득도상응적 HA 화 NA 기인편단。경과측서비대분석증실소분리병독위 H6N6아형금류감병독。귀주압군중존재 H6N6아형금류감병독，위진일보연구기기원화치병성제공료중요의거。
The present study was aimed to explore the presence and spread of H6N6 subtype influenza virus in birds in order to provide a basic dynamic of monitoring or prevention and control of avian influenza outbreaks in Guizhou.The 50 cloacal swabs were collected from duck farms in Sansui of Guizhou province,the virus was isolated and inoculated to 9 day chicken-embryos.By hemagglutinin (HA)and hemagglutination inhibition (HI)tests,the virus is neither a Newcastle disease virus,nor is H5,H7 and H9 subtypes of avian influenza virus.The viral RNA was extracted to amplify the corresponding HA and NA gene fragments by universal primers of avian influenza vi-rus HA and NA genes.These results suggested that the strain was H6N6 avian influenza virus by comparison with other strains.This study proved that the H6N6 subtype influenza virus exists in ducks of Guizhou,it provides an important basis to further study its origins and pathogenicity.