CAJ | 학술논문

目的：观察栀子-连翘药对配伍前后对发热型大鼠清热、利胆方面的影响，证明其配伍可以明显增强清热、利胆作用。方法：采用背部皮下注射干酵母混悬液法建立大鼠发热模型。制备栀子、连翘、栀子-连翘药对水提取物冻干粉，临用前水溶解灌胃给药。将SD大鼠随机分为正常组、模型组、阳性药对照组及中药给药组，中药给药组分为栀子、连翘单药给药组及栀子-连翘药对给药组。中药提取物溶液浓度为10 mL·kg-1（相当于单味药生药量3 g·kg-1），阳性药对照组给药浓度为10 mL·kg-1，空白组、模型组灌胃给予等量的生理盐水。除正常组大鼠外，各组大鼠背部皮下注射15%的干酵母悬液10 mL·kg-1，建立大鼠发热模型。电子体温计记录大鼠0、1、4、5、6、7、8 h体温，同时收集1-1、1-2、2-4、4-6、6-8、8-12、12-24 h胆汁，观察配伍前后发热大鼠的体温和胆汁量变化。结果：栀子、连翘及其药对配伍均具有一定的降低大鼠体温的作用，二药配伍后降温效果优于单用栀子或连翘；栀子-连翘药对能够降低发热大鼠的体温（P<0.05或P<0.01），对正常大鼠的体温有一定影响；栀子与连翘配伍具有一定的促进发热大鼠胆汁分泌的作用，二药配伍后胆汁分泌效果明显优于单用栀子（P<0.05）。结论：栀子与连翘配伍在大鼠发热模型上的降温能力优于单药应用，但无显著性差异；而在促进胆汁分泌方面具有显著性差异，说明该药对配伍后利胆作用增强。
목적：관찰치자-련교약대배오전후대발열형대서청열、리담방면적영향，증명기배오가이명현증강청열、리담작용。방법：채용배부피하주사간효모혼현액법건립대서발열모형。제비치자、련교、치자-련교약대수제취물동간분，림용전수용해관위급약。장SD대서수궤분위정상조、모형조、양성약대조조급중약급약조，중약급약조분위치자、련교단약급약조급치자-련교약대급약조。중약제취물용액농도위10 mL·kg-1（상당우단미약생약량3 g·kg-1），양성약대조조급약농도위10 mL·kg-1，공백조、모형조관위급여등량적생리염수。제정상조대서외，각조대서배부피하주사15%적간효모현액10 mL·kg-1，건립대서발열모형。전자체온계기록대서0、1、4、5、6、7、8 h체온，동시수집1-1、1-2、2-4、4-6、6-8、8-12、12-24 h담즙，관찰배오전후발열대서적체온화담즙량변화。결과：치자、련교급기약대배오균구유일정적강저대서체온적작용，이약배오후강온효과우우단용치자혹련교；치자-련교약대능구강저발열대서적체온（P<0.05혹P<0.01），대정상대서적체온유일정영향；치자여련교배오구유일정적촉진발열대서담즙분비적작용，이약배오후담즙분비효과명현우우단용치자（P<0.05）。결론：치자여련교배오재대서발열모형상적강온능력우우단약응용，단무현저성차이；이재촉진담즙분비방면구유현저성차이，설명해약대배오후리담작용증강。
This study was aimed to observe the antipyresis and cholagogue effect of theFructus Gardeniae-Fructus Forsythiaeherb couple in order to explore the possible pharmacodynamic mechanism. Dry yeast suspension was subcutaneously injected into the back of rat to establish the fever model. The freeze-dried powders ofF. Gardeniae,F. Forsythiae, andF. Gardeniae-F. Forsythiaeherb couple were prepared. They were dissolved in the water for intragastric administration. SD rats were randomly divided into the normal group, model group, positive control group and Chinese medicine group. The Chinese medicine group was subdivided into the group ofF. Gardeniae,F. Forsythiae, andF. Gardeniae-F. Forsythiaeherb couple. The concentration of herbal water extract was 10 mL·kg-1 (which equaled to 3 g·kg-1 of a single crude herb). The concentration of positive control was 10 mL·kg-1. Intragastric administration of equal amount of normal saline was given to the blank group and the model group. Except rats in the normal group, rats in other groups were subcutaneously injected with 10 mL·kg-1 of 15% dry yeast suspension on the back of to establish the fever model. Electronic thermometer was used to record the body temperature of rats at 0, 1, 4, 5, 6, 7 and 8 h after the injection, respectively. Meanwhile, bile was collected from 1-1, 1-2, 2-4, 4-6, 6-8, 8-12, 12-24 h, respectively. Observation was given on changes of body temperature and bile amount of rat. The results showed thatF. Gardeniae,F. Forsythiae, andF. Gardeniae- F. Forsythiaeherb couple had certain effect to reduce the body temperature of rats. The temperature-reducing effect of the combination of both herbs was better than a single herb. TheF. Gardeniae -F. Forsythiae herb couple can reduce the body temperature of fever rat (P < 0.05, orP < 0.01). It had a little effect on the body temperature of normal rat. TheF. Gardeniae - F. Forsythiaeherb couple can promote the bile secretion, which was better than the single using of F. Gardeniae (P < 0.05). It was concluded that theF. Gardeniae - F. Forsythiaeherb couple had better temperature-reducing effect than the using of a single herb; however, there was no significant difference. But it had obvious effect on the promotion of bile secretion, which indicated the strengthening of cholagogue effect.