CAJ | 학술논문

为了解四川地区猪环曲病毒（PToV）的发病状况，针对 PToV 的保守区域 S 基因设计了一对特异性引物，建立检测 PToV 的 RT-PCR 方法，并对其敏感性、特异性和重复性进行了检测。结果显示，仅猪环曲病毒阳性模板可扩增得到约569 bp 大小的目的条带。敏感性试验显示，该方法能检测到的最低核酸含量为15 pg。对采集于12个猪场27个腹泻样本和29个临床健康的样品进行了检测。结果显示，腹泻样本中检出率为33．3％（9／27），而在健康样本中检出率为6．9％（2／29）。以上结果表明本研究成功建立了一种敏感度高、特异性强、重复性良好的用于 PToV 的 RT-PCR 方法。
위료해사천지구저배곡병독（PToV）적발병상황，침대 PToV 적보수구역 S 기인설계료일대특이성인물，건립검측 PToV 적 RT-PCR 방법，병대기민감성、특이성화중복성진행료검측。결과현시，부저배곡병독양성모판가확증득도약569 bp 대소적목적조대。민감성시험현시，해방법능검측도적최저핵산함량위15 pg。대채집우12개저장27개복사양본화29개림상건강적양품진행료검측。결과현시，복사양본중검출솔위33．3％（9／27），이재건강양본중검출솔위6．9％（2／29）。이상결과표명본연구성공건립료일충민감도고、특이성강、중복성량호적용우 PToV 적 RT-PCR 방법。
To understand the infection of porcine torovirus(PToV)in pigs of Sichuan province,the RT-PCR meth-od for detecting PToV was developed based on the specific primers.And its sensitivity,specificity and repeatability were tested.The results indicated that only PToV was successfully amplified with a target fragment of 569 bp in size.Testing of the sensitivity of RT-PCR indicated that the lowest detection limit was 15 pg nuclear acids.A total of 27 diarrheal feces samples and 29 healthy feces samples were collected from Sichuan province for PToV detec-tion,the results exhibited that the positive rate of PToV was 33.3% from diarrheal samples and 6.9% from healthy samples.The above result suggested that we successfully established the RT-PCR method with high sensi-tivity,strong specificity and good repeatability for PToV detection.