CAJ | 학술논문

为了解广西鸭圆环病毒（DuCV）流行毒株的遗传变异情况，进一步为研制鉴别诊断 DuCV 方法和制定科学防控措施奠定基础。采用 PCR 方法对采自广西不同地区的阳性鸭组织样品进行了全基因组扩增和测序，共获得14个完整的 DuCV 全基因组序列。与 GenBank 中登录的52个国内外 DuCV 毒株进行遗传进化分析及同源性比较。结果表明，当前广西同时存在基因1．1亚型、基因1．2亚型和基因2．1亚型3种不同的 DuCV 毒株在流行，其中基因1．1亚型和1．2亚型为优势流行毒株。
위료해엄서압원배병독（DuCV）류행독주적유전변이정황，진일보위연제감별진단 DuCV 방법화제정과학방공조시전정기출。채용 PCR 방법대채자엄서불동지구적양성압조직양품진행료전기인조확증화측서，공획득14개완정적 DuCV 전기인조서렬。여 GenBank 중등록적52개국내외 DuCV 독주진행유전진화분석급동원성비교。결과표명，당전엄서동시존재기인1．1아형、기인1．2아형화기인2．1아형3충불동적 DuCV 독주재류행，기중기인1．1아형화1．2아형위우세류행독주。
To understand the genetic varition of DuCV in Guangxi,and further develop of diagnosis methods for identification of DuCV,the DuCV-positive tissue samples of ducks collected from various areas of Guan-gxi were amplified by PCR for full-length genome cloning and sequencing.14 complete genomic sequences were obtained,and subsequently,serviced for phylogenetic analysis and homology comparison with other 52 genomic sequences of DuCV in Genbank.The results showed that DuCV Guangxi isolates are divided into 3 lineages,genotypes 1.1,1.2,and 2.1.In addition,genotypes 1.1 and 1.2 are dominant.