实用医技杂志
實用醫技雜誌
실용의기잡지
Journal of Practical Medical Techniques
2015年
9期
915-918
,共4页
谢优%史振鸿%熊货杰%谭忠%罗义欢%陈罡%党裔武
謝優%史振鴻%熊貨傑%譚忠%囉義歡%陳罡%黨裔武
사우%사진홍%웅화걸%담충%라의환%진강%당예무
癌,肝细胞%微RNAs%Hep G2细胞%受体,肿瘤坏死因子,成员6b
癌,肝細胞%微RNAs%Hep G2細胞%受體,腫瘤壞死因子,成員6b
암,간세포%미RNAs%Hep G2세포%수체,종류배사인자,성원6b
Carcinoma,hepatocellur%Micro RNAs%Hep G2 cells%Receptors,tumor necrosis factor,member 6b
目的：研究微小RNA-152（miR-152）与其潜在靶基因DcR3在肝癌组织中表达的关系及miR-152在肝癌细胞系HepG2中对DcR3及相关信号通路的影响。方法使用实时荧光定量聚合酶链反应（RT-qPCR）检测89例肝癌组织中的miR-152表达水平，并使用免疫组织化学检测其DcR3蛋白表达水平，对比二者相关性；使用生物信息学软件对二者潜在靶向关系进行预测；将miR-152抑制剂及模拟物转染至肝癌细胞系HepG2中，使用蛋白印迹法测DcR3蛋白及相关基因Wnt-1，DNMT-1，ERK1/2及AKT表达情况。结果肝癌组织中，miR-152与DcR3蛋白表达水平呈负相关（r=-0.347，P=0.002）；软件预测发现miR-152与DcR33′-UTR序列呈互补关系；使用miR-152模拟物后，DcR3蛋白水平明显下降，同时Wnt-1，DNMT-1，p-ERK1/2及p-AKT表达也明显下降。结论在肝癌中，DcR3可能为miR-152的靶基因，miR-152可通过靶向DcR3而影响相应的增殖及凋亡通路。
目的：研究微小RNA-152（miR-152）與其潛在靶基因DcR3在肝癌組織中錶達的關繫及miR-152在肝癌細胞繫HepG2中對DcR3及相關信號通路的影響。方法使用實時熒光定量聚閤酶鏈反應（RT-qPCR）檢測89例肝癌組織中的miR-152錶達水平，併使用免疫組織化學檢測其DcR3蛋白錶達水平，對比二者相關性；使用生物信息學軟件對二者潛在靶嚮關繫進行預測；將miR-152抑製劑及模擬物轉染至肝癌細胞繫HepG2中，使用蛋白印跡法測DcR3蛋白及相關基因Wnt-1，DNMT-1，ERK1/2及AKT錶達情況。結果肝癌組織中，miR-152與DcR3蛋白錶達水平呈負相關（r=-0.347，P=0.002）；軟件預測髮現miR-152與DcR33′-UTR序列呈互補關繫；使用miR-152模擬物後，DcR3蛋白水平明顯下降，同時Wnt-1，DNMT-1，p-ERK1/2及p-AKT錶達也明顯下降。結論在肝癌中，DcR3可能為miR-152的靶基因，miR-152可通過靶嚮DcR3而影響相應的增殖及凋亡通路。
목적：연구미소RNA-152（miR-152）여기잠재파기인DcR3재간암조직중표체적관계급miR-152재간암세포계HepG2중대DcR3급상관신호통로적영향。방법사용실시형광정량취합매련반응（RT-qPCR）검측89례간암조직중적miR-152표체수평，병사용면역조직화학검측기DcR3단백표체수평，대비이자상관성；사용생물신식학연건대이자잠재파향관계진행예측；장miR-152억제제급모의물전염지간암세포계HepG2중，사용단백인적법측DcR3단백급상관기인Wnt-1，DNMT-1，ERK1/2급AKT표체정황。결과간암조직중，miR-152여DcR3단백표체수평정부상관（r=-0.347，P=0.002）；연건예측발현miR-152여DcR33′-UTR서렬정호보관계；사용miR-152모의물후，DcR3단백수평명현하강，동시Wnt-1，DNMT-1，p-ERK1/2급p-AKT표체야명현하강。결론재간암중，DcR3가능위miR-152적파기인，miR-152가통과파향DcR3이영향상응적증식급조망통로。
Objective To investigate the correlation between microRNA-152(miR-152) and the expression of its potential target gene DcR3 in hepatocellular carcinoma (HCC) tissues, and to study the effect of miR-152 on DcR3 level and relevant cell signaling pathway in HepG2 HCC cells. Methods Real time RT-qPCR was performed to detect the miR-152 level in 89 cases of HCC tissues, and immunohistochemistry was used to examine the expression of DcR3 protein in the same cohort. The relationship between miR-152 and DcR3 was analyzed. The potential targeting relationship between miR-152 and DcR3 was predicted through online bioinformatics software. MiR-152 inhibitor and miR-152 mimic were transfected into HCC HepG2 cells and DcR3 protein as well as Wnt-1, DNMT-1, ERK1/2 and AKT were detected with western blot. Results In HCC tissues, miR-152 was inversely related to DcR3 protein expression (r=-0.347, P=0.002). The complementarity between miR-152 and the sequence of DcR3 3′-UTR was confirmed by prediction software. Furthermore, after transfection of miR-152 mimic, the protein level of DcR3 reduced, as well as Wnt-1, DNMT-1, p-ERK1/2 and p-AKT. Conclusion In HCC, DcR3 could be the target gene of miR-152. MiR-152 could influence relevant pathways of cell proliferation and apoptosis.
