CAJ | 학술논문

目的：通过连续20 d对雄性小鼠灌胃染毒3，4亚甲基二氧基甲基苯丙胺（MDMA）后，探究MDMA对雄性小鼠睾丸组织细胞微核率及染色体畸变率的影响。方法将雄性小鼠随机数字表法分为 MDMA 低（5.0 mg/kg）、中（10.0 mg/kg）、高（20.0 mg/kg）3个染毒剂量组，采用0.9%氯化钠注射液做阴性对照，每日染毒1次。于末次给药后第2天，取小鼠睾丸组织细胞，采用常规微核（MN）试验，检测小鼠睾丸细胞微核率的改变；同时采用染色体畸变试验探究MDMA对小鼠睾丸细胞染色体畸变率的影响。结果微核试验结果表明MDMA高剂量与低剂量组小鼠睾丸细胞微核率及阴性对照组比较，差异有统计学意义（P<0.01）。染色体畸变试验结果中，MDM高、中剂量组染色体畸变率分别与阴性对照组比较，差异有统计学意义（P<0.05），高剂量组与中、低剂量组染色体畸变率比较差异有统计学意义（P<0.05）。结论高剂量组MDMA能诱导小鼠睾丸组织细胞微核率增加，高剂量组及中剂量组可以使小鼠睾丸细胞染色体畸变率增高，说明其对雄性小鼠睾丸遗传物质有一定的损伤效应。
목적：통과련속20 d대웅성소서관위염독3，4아갑기이양기갑기분병알（MDMA）후，탐구MDMA대웅성소서고환조직세포미핵솔급염색체기변솔적영향。방법장웅성소서수궤수자표법분위 MDMA 저（5.0 mg/kg）、중（10.0 mg/kg）、고（20.0 mg/kg）3개염독제량조，채용0.9%록화납주사액주음성대조，매일염독1차。우말차급약후제2천，취소서고환조직세포，채용상규미핵（MN）시험，검측소서고환세포미핵솔적개변；동시채용염색체기변시험탐구MDMA대소서고환세포염색체기변솔적영향。결과미핵시험결과표명MDMA고제량여저제량조소서고환세포미핵솔급음성대조조비교，차이유통계학의의（P<0.01）。염색체기변시험결과중，MDM고、중제량조염색체기변솔분별여음성대조조비교，차이유통계학의의（P<0.05），고제량조여중、저제량조염색체기변솔비교차이유통계학의의（P<0.05）。결론고제량조MDMA능유도소서고환조직세포미핵솔증가，고제량조급중제량조가이사소서고환세포염색체기변솔증고，설명기대웅성소서고환유전물질유일정적손상효응。
Objective To study the effects of 3,4-methylenedioxymethamphetamine (MDMA) on micronucleus frequency and chromosomal aberration ratios of male mouse testicle cells. Methods Healthy adult male rats were randomly divided into four groups. Different doses of MDMA (0, 5, 10 and 20 mg/kg) were administrated to the rats every days for 20 days. The rats were killed after the last test substance given for 24 h. Each rat′s micronucleus frequency and chromosomal aberration ratio of testicle cell were detected by using micronucleus test and chromosomal aberration test. Results The micronucleus frequencies of rats testicle cell in high dose group was significantly different from the control group and low dose group(P<0.01). With the control group, the chromosomal aberration ratios in testis of rats in high and middle dose group increased, the difference was significant (P<0.05), that in high dose group was also significantly different from the low and middle dose group (P<0.05). Conclusion It was suggested that MDMA might damage genetic material of rat testicle cells.