CAJ | 학술논문

通过培养基 LB、改良 LB、改良 LB＋微量盐、改良 Minca 和2×YT＋10 g／L 葡萄糖5种培养基进行筛选，设定培养基初始 pH 分别为6．0、6．5、7．0和7．5四个梯度进行蛋白表达，用全自动微生物生长仪每隔30 min 测定菌液的 OD 值，最终确定2×YT＋5 g／L 的葡萄糖生长菌数较多，细菌生长速度快，pH7．0时蛋白表达量较高，并且通过细菌生长曲线得出最佳诱导时机为细菌培养开始后3．0 h～3．5 h，诱导时长4．0 h，溶氧量控制在30％，为 ETEC 大肠埃希菌高密度发酵工艺的研究提供了基础数据。
통과배양기 LB、개량 LB、개량 LB＋미량염、개량 Minca 화2×YT＋10 g／L 포도당5충배양기진행사선，설정배양기초시 pH 분별위6．0、6．5、7．0화7．5사개제도진행단백표체，용전자동미생물생장의매격30 min 측정균액적 OD 치，최종학정2×YT＋5 g／L 적포도당생장균수교다，세균생장속도쾌，pH7．0시단백표체량교고，병차통과세균생장곡선득출최가유도시궤위세균배양개시후3．0 h～3．5 h，유도시장4．0 h，용양량공제재30％，위 ETEC 대장애희균고밀도발효공예적연구제공료기출수거。
The five kinds of media,LB,improved LB,improved LB+ trace salt solution,improved Minca and 2×YT+10 g/L glucose were screened,and the initial pH of medium were set as 6.0,6.5,7.0 and 7.5 for protein expression.With automatic microorganism growth instrument,OD values of bacterial liquid were measured every half an hour.Finally 2 × YT + 5 g/L glucose and pH7.0 were confirmed for the growth of bacterial protein expression.The optimal induction time was in 3.0 h-3.5 h after culture by bac-terial growth curve,induction time 4.0 h,oxygen control at 30%,for ETEC E.coli to provide basic data for the research of high density fermentation.