CAJ | 학술논문

目的观察microRNA-21 ( miR-21 )对裸鼠人胆管癌细胞移植瘤生长及上皮间质转化( EMT)进程的影响. 方法在两组裸鼠侧腹部皮下注射对数生长期的胆管癌细胞QBC939和稳定表达miR-21的QBC939-miR-21细胞,建立裸鼠移植瘤模型. 采用Western blot、免疫组化及实时定量RT-PCR法检测EMT的上皮细胞标志物 E-钙粘蛋白( E-cadher-in)和间质标志物N-钙粘蛋白( N-cadherin)、波形蛋白( Vim-entin)的表达. 结果 QBC939-miR-21 组移植瘤体积大于QBC939组( P<0. 05 ) ,且E-cadherin相对表达量降低, Vim-entin、N-cadherin相对表达量增加. 结论 miR-21促进了裸鼠胆管癌细胞移植瘤生长和 EMT 进程,为进一步研究 miR-21在胆管癌中的功能及作用机制奠定了基础.
목적 관찰microRNA-21 ( miR-21 )대라서인담관암세포이식류생장급상피간질전화( EMT)진정적영향. 방법재량조라서측복부피하주사대수생장기적담관암세포QBC939화은정표체miR-21적QBC939-miR-21세포,건립라서이식류모형. 채용Western blot、면역조화급실시정량RT-PCR법검측EMT적상피세포표지물 E-개점단백( E-cadher-in)화간질표지물N-개점단백( N-cadherin)、파형단백( Vim-entin)적표체. 결과 QBC939-miR-21 조이식류체적대우QBC939조( P<0. 05 ) ,차E-cadherin상대표체량강저, Vim-entin、N-cadherin상대표체량증가. 결론 miR-21촉진료라서담관암세포이식류생장화 EMT 진정,위진일보연구 miR-21재담관암중적공능급작용궤제전정료기출.
Objective To observe the effects of microRNA-21 ( miR-21 ) on the epithelial-mesenchymal transition ( EMT) of human cholangiocarcinoma xenografts in nude mice. Methods QBC-939 and QBC939-miR-21 cholan-giocarcinoma cells were subcutaneously injected into two groups of nude mice respectively as xenografts, expressions of E-cadherin, N-cadherin and Vimentin were detected by Western blot ,RT-PCR and immunohistochemical analy-sis. Results Compared with group QBC939,group QBC939-miR-21 xenografts showed the greater tumor weight and rapider growth,expression of E-cadherin decreased,whereas the protein expressions of Vimentin and N-cadherin increased. Conclusion miR-21 overexpression enhances tumor growth, which promotes EMT phenotype and the foundation for further study of miR-21 function in cholangiocarcinoma and mechanism is established.