安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
Acta Universitatis Medicinalis Anhui
2015年
10期
1426-1430
,共5页
树鼩%足底电击%条件恐惧记忆形成和抹除%明暗箱
樹鼩%足底電擊%條件恐懼記憶形成和抹除%明暗箱
수구%족저전격%조건공구기억형성화말제%명암상
tree shrew%foot-shock%fear conditioning memory formation and erasing%light/dark box
目的 探索足底电击诱导树鼩条件恐惧记忆模型的建立及抹除方法. 方法 测定在无刺激条件下,树鼩在明暗箱中的活动规律;然后用梯度刺激实验测定合适的足底电击电压;最后用合适的电压通过足底电击诱导并建立实验组树鼩条件恐惧记忆模型并抹除其记忆. 结果 正常条件下,树鼩(n=4)在暗箱中的停留时间明显多于明箱中的停留时间(P<0. 01);在两明箱中,足底电压梯度刺激实验显示,随着刺激电压的增加,树鼩(n=6)在有刺激的隔间中的停留时间逐渐减少. 12 V以后树鼩在有刺激隔间与无刺激隔间的停留时间差异有统计学意义[12 V(P <0. 05)、16 V(P <0. 01)、20 V(P<0. 01)];实验组(n=4)暗箱用16 V刺激电压足底电击能够形成明显的恐惧记忆( P<0. 001 ). 在形成明显的恐惧记忆后,在明箱中给予相应的足底电击4 d,实验组(n=4)树鼩在明箱中停留的时间逐渐减少,与对照组比较差异无统计学意义. 结论 树鼩更趋于停留在暗箱中;16 V为较为适合的足底电击电压;条件恐惧记忆能够被诱导并且被新的刺激完全抹除.
目的 探索足底電擊誘導樹鼩條件恐懼記憶模型的建立及抹除方法. 方法 測定在無刺激條件下,樹鼩在明暗箱中的活動規律;然後用梯度刺激實驗測定閤適的足底電擊電壓;最後用閤適的電壓通過足底電擊誘導併建立實驗組樹鼩條件恐懼記憶模型併抹除其記憶. 結果 正常條件下,樹鼩(n=4)在暗箱中的停留時間明顯多于明箱中的停留時間(P<0. 01);在兩明箱中,足底電壓梯度刺激實驗顯示,隨著刺激電壓的增加,樹鼩(n=6)在有刺激的隔間中的停留時間逐漸減少. 12 V以後樹鼩在有刺激隔間與無刺激隔間的停留時間差異有統計學意義[12 V(P <0. 05)、16 V(P <0. 01)、20 V(P<0. 01)];實驗組(n=4)暗箱用16 V刺激電壓足底電擊能夠形成明顯的恐懼記憶( P<0. 001 ). 在形成明顯的恐懼記憶後,在明箱中給予相應的足底電擊4 d,實驗組(n=4)樹鼩在明箱中停留的時間逐漸減少,與對照組比較差異無統計學意義. 結論 樹鼩更趨于停留在暗箱中;16 V為較為適閤的足底電擊電壓;條件恐懼記憶能夠被誘導併且被新的刺激完全抹除.
목적 탐색족저전격유도수구조건공구기억모형적건립급말제방법. 방법 측정재무자격조건하,수구재명암상중적활동규률;연후용제도자격실험측정합괄적족저전격전압;최후용합괄적전압통과족저전격유도병건립실험조수구조건공구기억모형병말제기기억. 결과 정상조건하,수구(n=4)재암상중적정류시간명현다우명상중적정류시간(P<0. 01);재량명상중,족저전압제도자격실험현시,수착자격전압적증가,수구(n=6)재유자격적격간중적정류시간축점감소. 12 V이후수구재유자격격간여무자격격간적정류시간차이유통계학의의[12 V(P <0. 05)、16 V(P <0. 01)、20 V(P<0. 01)];실험조(n=4)암상용16 V자격전압족저전격능구형성명현적공구기억( P<0. 001 ). 재형성명현적공구기억후,재명상중급여상응적족저전격4 d,실험조(n=4)수구재명상중정류적시간축점감소,여대조조비교차이무통계학의의. 결론 수구경추우정류재암상중;16 V위교위괄합적족저전격전압;조건공구기억능구피유도병차피신적자격완전말제.
Objective To investigate the way of fear conditioning memory model evoked and erased by foot-shock in tree shrew. Methods First, detect the tree shrew activities regularly in light/dark box. Second, test a suitable voltage degree of foot shock on tree shrew. Third, investigate the memory formation and erasing of fear conditioning on tree shrew of trial group. Results The duration of tree shrew (n=4) stay in the dark-box was significantly lon-ger than that of in the light box (P<0. 01) in normal condition. In the same environment of two light boxes, given different voltage degrees, the durations of tree shrew (n=6) stay in the stimulating chamber gradually reduced and the durations of tree shrew stay had significant difference between stimulatus chamber and no stimulatus chamber when the stimulus voltage up to 12 V ( P<0. 05 ) , 16 V ( P<0. 01 ) and 20 V ( P<0. 01 ) . The animal of trial group ( n=4 ) could build up the fear conditioning memory of the dark box with the stimulus of 16 V foot-shock in the dark box ( P<0. 001 ) . After formation of the fear conditioning memory, the same stimulus in light box ap-peared for 4 days. The durations of tree shrew stay in trial group (n=4) decreased in light box, and there was no significant difference between the trial group and the control group. Conclusion Tree shrew prefers to stay in the dark box. The suitable voltage for foot-shock on tree shrew is 16 V. The fear conditioning memory can be evoked and erased by foot-shock.
