CAJ | 학술논문

为建立膜连蛋白 A2（AnxA2）基因 SYBR Green Ⅰ实时荧光定量 PCR 检测方法，根据 Gen-Bank 中公布的 AnxA2基因序列设计并合成引物，经过条件优化，建立了 AnxA2 SYBR Green Ⅰ实时荧光定量 PCR 检测方法。该方法线性关系较好，以质粒标准品构建的标准曲线相关系数 R2为0．999；灵敏性好，比普通 PCR 灵敏100倍。应用建立的方法对随机选取的几种哺乳动物细胞进行检测。结果表明，建立的方法能成功检出不同细胞中 AnxA2含量。表明本研究建立的 AnxA2 SYBR Green Ⅰ实时荧光定量PCR 检测方法灵敏性高，重复性好，可用于 AnxA2的检测及定量分析。
위건립막련단백 A2（AnxA2）기인 SYBR Green Ⅰ실시형광정량 PCR 검측방법，근거 Gen-Bank 중공포적 AnxA2기인서렬설계병합성인물，경과조건우화，건립료 AnxA2 SYBR Green Ⅰ실시형광정량 PCR 검측방법。해방법선성관계교호，이질립표준품구건적표준곡선상관계수 R2위0．999；령민성호，비보통 PCR 령민100배。응용건립적방법대수궤선취적궤충포유동물세포진행검측。결과표명，건립적방법능성공검출불동세포중 AnxA2함량。표명본연구건립적 AnxA2 SYBR Green Ⅰ실시형광정량PCR 검측방법령민성고，중복성호，가용우 AnxA2적검측급정량분석。
In order to establish AnxA2 SYBR Green I real-time PCR,one pair of primers were designed and synthesized based on the published gene sequence of AnxA2 in GenBank.After optimizing the reaction con-ditions,AnxA2 SYBR Green I real-time PCR was established with good linear relationship,and the stand-ard calibration curve correlation coefficient R2 was 0 .999 for standard plasmid.It also had good sensitivity, about 100 times more sensitive than conventional PCR.We also detected several randomly selected mam-malian cells with this method.The results showed that the method could successfully detect the content of AnxA2 in different cells.It proved that the established AnxA2 SYBR Green I real-time PCR was of high sensitivity,good reproducibility and could be used in the detection of AnxA2 and quantitative analysis.