CAJ | 학술논문

表面活性素（ surfactin）中的C15组分是表面活性素Surfactin异构体混合物中的重要活性组分，在表面活性、溶血活性、抗病毒活性和抑菌活性方面都有突出的表现。考察培养基pH、碳源、氮源、碳氮比、金属离子以及氨基酸等主要参数对枯草芽胞杆菌发酵产物surfactin中C15组分的影响，利用优化后的培养基（葡萄糖20 g／L、NH4 NO32 g／L、K2 HPO43 g／L、NaH2 PO410 g／L、MgSO40?02 g／L、Leu 1 g／L和pH 8?5），枯草芽胞杆菌BS 37发酵24 h， surfactin总产量达750 mg／L，其中C15组分达510 mg／L，占surfactin总量的68％。
표면활성소（ surfactin）중적C15조분시표면활성소Surfactin이구체혼합물중적중요활성조분，재표면활성、용혈활성、항병독활성화억균활성방면도유돌출적표현。고찰배양기pH、탄원、담원、탄담비、금속리자이급안기산등주요삼수대고초아포간균발효산물surfactin중C15조분적영향，이용우화후적배양기（포도당20 g／L、NH4 NO32 g／L、K2 HPO43 g／L、NaH2 PO410 g／L、MgSO40?02 g／L、Leu 1 g／L화pH 8?5），고초아포간균BS 37발효24 h， surfactin총산량체750 mg／L，기중C15조분체510 mg／L，점surfactin총량적68％。
C15 Surfactin component is an important active group of surfactin variants;it has outstanding performance in surface activity,hemolytic activity and antibacterial activity. We studied the effect of pH, carbon source, nitrogen source, C/N ratio, metal ion and amino acid of the culture medium on C15 components accumulation.After 24 h fermentation with the optimized medium(glucose 20 g/L,NH4NO3 2 g/L,K2HPO4 3 g/L,NaH2PO4 10 g/L,MgSO4 0.02 g/L,Leu 1 g/L and pH 8.5),the titer of surfactin variants was 750 mg/L, in which C15 component was 510 mg/L, accounting for 68% of total surfactin produced by Bacillus subtilis BS 37.