CAJ | 학술논문

微生物发酵和酶转化法是工业上制备三磷酸腺苷（ ATP ）的有效途径。以腺嘌呤为关键底物，用冻融通透化处理的产氨棒杆菌细胞转化制备ATP，用高效液相色谱（HPLC）法检测ATP含量，考察各种转化条件对ATP产率的影响，确定最优转化条件：菌体用量40 g／L，底物6 g／L，葡萄糖60 g／L，MgSO415 mmol／L，KH2 PO4120 mmol／L，反应液pH 7?4，反应温度35℃。在最优转化条件下，ATP产率达到85?00％，比优化前提高了58％，细胞用量大幅度降低，优化条件稳定可行。
미생물발효화매전화법시공업상제비삼린산선감（ ATP ）적유효도경。이선표령위관건저물，용동융통투화처리적산안봉간균세포전화제비ATP，용고효액상색보（HPLC）법검측ATP함량，고찰각충전화조건대ATP산솔적영향，학정최우전화조건：균체용량40 g／L，저물6 g／L，포도당60 g／L，MgSO415 mmol／L，KH2 PO4120 mmol／L，반응액pH 7?4，반응온도35℃。재최우전화조건하，ATP산솔체도85?00％，비우화전제고료58％，세포용량대폭도강저，우화조건은정가행。
In industrial process, ATP can be produced by fermentation with microbial strains or biotransformation with enzymes. In this work,permeable Corynebacterium ammoniagenes cells with frozen?thawed processing were used to synthesize ATP using adenine as the primary substrate. The preparation conditions were studied experimentally and ATP concentration in the culture broth was detected by high performance liquid chromatography (HPLC). The optimum condition was observed,i.e.,wet cells 40 g/L,adenine 6 g/L,glucose 60 g/L,MgSO4 15 mmol/L,KH2PO4 120 mmol/L,pH 7?4 and the reaction temperature at 35 ℃.As a result,adenine was biotransformed successfully into ATP with the yield about 85?00%,which was 58% higher than that before the optimization. The amount of cells needed in the present process was much less than those without frozen?thawed processing. The optimum conditions obtained were stable and feasible.