CAJ | 학술논문

目的探讨4种主要的细胞色素P450酶在非酒精性脂肪肝( NAFLD)发病进程中的表达变化. 方法建立大鼠NAFLD的病理模型,并分别测定肝匀浆中谷草转氨酶(AST)、谷丙转氨酶(ALT)和三酰甘油(TG)等生化指标.采用Western blot法检测在各个病理时期CYP1A2、CYP2D6、CYP2E1和CYP3A1的表达水平的变化.结果随着NAFLD严重程度的增加,AST与ALT的活性显著上升( P<0. 01 ) , TG的水平则在重度脂肪肝时期之前上升,而后显著下降( P<0. 01).CYP1A2和CYP2D6的表达水平在发病进程中总体呈下降趋势, CYP3A1 虽总体下调,但幅度不大,而CYP2E1的表达水平则明显上升( P <0. 01 ). 结论在NAFLD 的发病进程中 CYP1A2、 CYP2D6、 CYP3A1 和CYP2E1的表达水平发生明显的改变,实验结果可以为内科医师针对NAFLD患者的用药提供指导.
목적 탐토4충주요적세포색소P450매재비주정성지방간( NAFLD)발병진정중적표체변화. 방법 건립대서NAFLD적병리모형,병분별측정간균장중곡초전안매(AST)、곡병전안매(ALT)화삼선감유(TG)등생화지표.채용Western blot법검측재각개병리시기CYP1A2、CYP2D6、CYP2E1화CYP3A1적표체수평적변화.결과 수착NAFLD엄중정도적증가,AST여ALT적활성현저상승( P<0. 01 ) , TG적수평칙재중도지방간시기지전상승,이후현저하강( P<0. 01).CYP1A2화CYP2D6적표체수평재발병진정중총체정하강추세, CYP3A1 수총체하조,단폭도불대,이CYP2E1적표체수평칙명현상승( P <0. 01 ). 결론 재NAFLD 적발병진정중 CYP1A2、 CYP2D6、 CYP3A1 화CYP2E1적표체수평발생명현적개변,실험결과가이위내과의사침대NAFLD환자적용약제공지도.
Objective To study the alterations of cytochrome P450 enzymes in rats liver with progressive stages of non-alcoholic fatty liver disease ( NAFLD) . Methods NAFLD models were established. Activities of hepatic as-partate aminotransferase ( AST) , alanine aminotransferase ( ALT) and levels of triglyceride ( TG) were measured by assay kit. The CYP1A2, CYP2D6, CYP2E1 and CYP3A1 protein expressions were detected by Western blot a-nalysis. Results Higher activities of ALT and AST were detected(P<0. 01)as the severity of NAFLD increased. TG levels increased remarkably in the range from normal to serious steatosis, and then decreased dramatically. Pro-tein expressions of CYP1 A2 , CYP2 D6 and CYP3 A1 tended to decrease with NAFLD progression while CYP2 E1 was increased consecutively. Conclusion These results suggest that significant and novel changes occur in hepatic P450 expression during progressive stages of NAFLD. It may provide a valuable framework for physicians when de-termining the pharmacotherapeutic options and dosing regimens to patients with this disease.