安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
Acta Universitatis Medicinalis Anhui
2015年
10期
1390-1393
,共4页
李保山%查小雪%朱德发%康冬梅
李保山%查小雪%硃德髮%康鼕梅
리보산%사소설%주덕발%강동매
甲状腺功能减退症%甲状腺素%多奈哌齐%syntaxin-1%海马
甲狀腺功能減退癥%甲狀腺素%多奈哌齊%syntaxin-1%海馬
갑상선공능감퇴증%갑상선소%다내고제%syntaxin-1%해마
hypothyroidism%thyroine%dozepezil%syntaxin-1%hippocampus
目的 观察甲状腺素( T4 )联合多奈哌齐对成年期甲状腺功能减退症(甲减)大鼠的海马内突触前膜相关蛋白1 (syntaxin-1)表达的影响,探讨甲减脑损伤及可能的恢复机制. 方法 44只SD大鼠随机分为4组:对照组、甲减组、T4替代治疗组及T4和多奈哌齐联合治疗组. 通过饮水给予丙基硫氧嘧啶0. 05%建立成年期大鼠甲减模型共6 周. 第5周开始,对照组腹腔注射等量生理盐水, T4替代治疗组每日给予左旋甲状腺素(L-T4) 6 μg/100 g 体重(BW)腹腔注射,联合治疗组每日给予6 μg/100g BW L-T4腹腔注射及饮用水中加入0. 005%多奈哌齐. 采用免疫组织化学法和West-ern blot法分析4 组大鼠海马syntaxin-1 的表达情况. 结果与对照组比较,甲减组大鼠血清三碘甲状腺原氨酸( T3 )、T4水平减低,促甲状腺激素(TSH)水平升高(P<0. 01),海马内syntaxin-1在CA1、CA3 各层及齿状区多形层( DG-PL)表达显著增加( P <0. 01 );T4 替代治疗后,血清 T3、T4 和TSH水平恢复至正常水平, syntaxin-1 蛋白的表达未完全恢复到对照组水平(P<0. 05);T4联合多奈哌齐治疗后syntax-in-1蛋白表达与对照组比较差异无统计学意义. 结论 T4联合多奈哌齐治疗可以修复成年期甲减导致的脑损伤.
目的 觀察甲狀腺素( T4 )聯閤多奈哌齊對成年期甲狀腺功能減退癥(甲減)大鼠的海馬內突觸前膜相關蛋白1 (syntaxin-1)錶達的影響,探討甲減腦損傷及可能的恢複機製. 方法 44隻SD大鼠隨機分為4組:對照組、甲減組、T4替代治療組及T4和多奈哌齊聯閤治療組. 通過飲水給予丙基硫氧嘧啶0. 05%建立成年期大鼠甲減模型共6 週. 第5週開始,對照組腹腔註射等量生理鹽水, T4替代治療組每日給予左鏇甲狀腺素(L-T4) 6 μg/100 g 體重(BW)腹腔註射,聯閤治療組每日給予6 μg/100g BW L-T4腹腔註射及飲用水中加入0. 005%多奈哌齊. 採用免疫組織化學法和West-ern blot法分析4 組大鼠海馬syntaxin-1 的錶達情況. 結果與對照組比較,甲減組大鼠血清三碘甲狀腺原氨痠( T3 )、T4水平減低,促甲狀腺激素(TSH)水平升高(P<0. 01),海馬內syntaxin-1在CA1、CA3 各層及齒狀區多形層( DG-PL)錶達顯著增加( P <0. 01 );T4 替代治療後,血清 T3、T4 和TSH水平恢複至正常水平, syntaxin-1 蛋白的錶達未完全恢複到對照組水平(P<0. 05);T4聯閤多奈哌齊治療後syntax-in-1蛋白錶達與對照組比較差異無統計學意義. 結論 T4聯閤多奈哌齊治療可以脩複成年期甲減導緻的腦損傷.
목적 관찰갑상선소( T4 )연합다내고제대성년기갑상선공능감퇴증(갑감)대서적해마내돌촉전막상관단백1 (syntaxin-1)표체적영향,탐토갑감뇌손상급가능적회복궤제. 방법 44지SD대서수궤분위4조:대조조、갑감조、T4체대치료조급T4화다내고제연합치료조. 통과음수급여병기류양밀정0. 05%건립성년기대서갑감모형공6 주. 제5주개시,대조조복강주사등량생리염수, T4체대치료조매일급여좌선갑상선소(L-T4) 6 μg/100 g 체중(BW)복강주사,연합치료조매일급여6 μg/100g BW L-T4복강주사급음용수중가입0. 005%다내고제. 채용면역조직화학법화West-ern blot법분석4 조대서해마syntaxin-1 적표체정황. 결과여대조조비교,갑감조대서혈청삼전갑상선원안산( T3 )、T4수평감저,촉갑상선격소(TSH)수평승고(P<0. 01),해마내syntaxin-1재CA1、CA3 각층급치상구다형층( DG-PL)표체현저증가( P <0. 01 );T4 체대치료후,혈청 T3、T4 화TSH수평회복지정상수평, syntaxin-1 단백적표체미완전회복도대조조수평(P<0. 05);T4연합다내고제치료후syntax-in-1단백표체여대조조비교차이무통계학의의. 결론 T4연합다내고제치료가이수복성년기갑감도치적뇌손상.
Objective To observe the effects of thyroxine(T4) and donepezil on hippocampal syntaxin-1 expression in adult hypothyroidism rats, and clarify the possible mechanism under the hypothyroidism-induced cognitive dys-function. Methods The Sprague-Dawley rats were randomly classified into four groups:normal control, hypothy-roid(PTU:0. 05%, add to the dringking water), hypothyroid receiving levothyroxine replacement therapy(6 μg/100 g body weight once daily, ip injection) , and a combination of both drugs ( levothyroxine:6 μg/100 g;done-pezil 0. 005%). Serum T3, T4 and TSH concentrations were obtained using a radioimmunoassay kit. Protein levels of syntaxin-1 were determined by immunohistochemistry and Western blot. Results Compared to the control, the serum T3 and T4 levels of the hypothyroid group rats were significantly decreased, serum TSH elevated, syntaxin-1 in the hippocampus was significantly increased in CA1, CA3 and DG-PL regions. After levothyroxine or combined with donepezil treatment, serum T3, T4 and TSH were returned to the normal. But treatment with levothyroxine a-lone failed to normalize the expression of syntaxin-1 . The level of syntaxin-1 was restored completely to the control group. Conclusion Our findings suggest that adult-onset hypothyroidism may induce increment of syntaxin-1 in the hippocampus, which could be restored preferably by T4 combined with donepezil treatment.
