CAJ | 학술논문

目的：探讨细胞块联合免疫组化技术在解决胸腔积液诊断中的应用价值。方法收集60例胸腔积液标本，应用脱落细胞石蜡包埋法制备细胞块，免疫组化法检测CK7、TTF-1、E-cadherin、CEA和Calretinin的表达。结果细胞块联合免疫组化技术的恶性诊断率高于常规离心涂片。 CK7、TTF-1、E-cadherin和CEA和Calretinin在胸腔积液肺腺癌细胞及反应性增生间皮细胞中的表达差异有统计学意义(P<0．05)。胸腔积液的肺腺癌细胞中CK7、TTF-1、E-cadherin和CEA呈高表达；增生性间皮细胞中Calretinin呈高表达。结论细胞块联合免疫组化技术在解决疑难胸腔积液鉴别诊断中具有重要的临床意义。
목적：탐토세포괴연합면역조화기술재해결흉강적액진단중적응용개치。방법수집60례흉강적액표본，응용탈락세포석사포매법제비세포괴，면역조화법검측CK7、TTF-1、E-cadherin、CEA화Calretinin적표체。결과세포괴연합면역조화기술적악성진단솔고우상규리심도편。 CK7、TTF-1、E-cadherin화CEA화Calretinin재흉강적액폐선암세포급반응성증생간피세포중적표체차이유통계학의의(P<0．05)。흉강적액적폐선암세포중CK7、TTF-1、E-cadherin화CEA정고표체；증생성간피세포중Calretinin정고표체。결론세포괴연합면역조화기술재해결의난흉강적액감별진단중구유중요적림상의의。
Purpose To investigate the application value in diagnosis of pleural effusion by cell block combined with immunohisto-chemistry. Methods 60 cases of pleural effusion were collected, and paraffin-embedded cell block was prepared and immunohisto-chemistry was used to detect the expression of CK7, TTF-1, E-cadherin, CEA and Calretinin. Results By use of cell block combined with immunohistochemistry, malignant detected rate was higher than that of the conventional centrifugal smear. There was statistical significance in the expression of CK7, TTF-1, E-cadherin, CEA and Calretinin between pleural effusion lung adenocarcinoma and reac-tive hyperplasia mesothelial cells (P<0. 05). CK7, TTF-1, E-cadherin and CEA were highly expressed in pleural effusion of lung ad-enocarcinoma cell. Calretinin was highly expressed in hyperplastic mesothelial cells. Conclusion Cell block and immunohistochemi-cal technique combination in the differential diagnosis of difficult pleural effusion has important clinical significance. It is worthy of popularization and further clinical application.