CAJ | 학술논문

目的：探讨苦瓜子有效成分RNA酶MC2(RNase MC2)在肝癌细胞生长中的作用及其机制。方法应用MTT、克隆形成和体内裸鼠成瘤实验研究RNase MC2对肝癌细胞增殖的影响,流式细胞技术检测RNase MC2对肝癌细胞周期的影响,电镜检测RNase MC2诱导的细胞自噬,Western blot法检测RNase MC2作用引起的蛋白变化。结果体内外实验结果显示RNase MC2显著抑制肝癌细胞增殖；上调p53和p21蛋白表达导致G2/M期阻滞；增加Beclin-1和LC3-Ⅱ蛋白表达诱导细胞自噬。RNase MC2在体内外均能增强Sorafenib对肝癌细胞的杀伤能力。结论苦瓜子有效成分RNase MC2可能通过细胞周期阻滞、诱导细胞自噬而显著抑制肝癌细胞的生长,并可增强Sorafenib对肝癌细胞的治疗效果。
목적：탐토고과자유효성분RNA매MC2(RNase MC2)재간암세포생장중적작용급기궤제。방법응용MTT、극륭형성화체내라서성류실험연구RNase MC2대간암세포증식적영향,류식세포기술검측RNase MC2대간암세포주기적영향,전경검측RNase MC2유도적세포자서,Western blot법검측RNase MC2작용인기적단백변화。결과체내외실험결과현시RNase MC2현저억제간암세포증식；상조p53화p21단백표체도치G2/M기조체；증가Beclin-1화LC3-Ⅱ단백표체유도세포자서。RNase MC2재체내외균능증강Sorafenib대간암세포적살상능력。결론고과자유효성분RNase MC2가능통과세포주기조체、유도세포자서이현저억제간암세포적생장,병가증강Sorafenib대간암세포적치료효과。
Purpose To determine the effect of RNase MC2 purified from momordica charantia on cell growth of hepatocellular carcino-ma ( HCC) and its underlying mechanism. Methods MTT, colony formation and nude mice model were used to examine the activity of RNase MC2 in cell proliferation. Cell cycle analysis was done by flow cytometry. Autophagy induced by RNase MC2 treatment was observed via transmission electron microscope. Western blot was performed to detect the RNase MC2-mediated changes of proteins. Re-sults In vitro and in vivo data showed that RNase MC2 markedly inhibited HCC cell proliferation, arrested cells at G2/M phase by in-creasing expression of p53 and p21, induced autophagy via upregulating Beclin-1 and LC3-Ⅱ. Furthermore, combination of RNase MC2 and Sorafenib exerted enhanced lethal effect on HCC cells. Conclusion RNase MC2 manifests significant antitumor activities and enhances the killing effect of Sorafenib in HCC via inducing cell cycle arrest and autophagy.