临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
Chinese Journal of Clinical and Experimental Pathology
2015年
8期
880-884
,共5页
肺肿瘤%FOXO3%肺癌干细胞%RT-PCR%Western blot
肺腫瘤%FOXO3%肺癌榦細胞%RT-PCR%Western blot
폐종류%FOXO3%폐암간세포%RT-PCR%Western blot
lung neoplasms%FOXO3%cancer stem cells%RT-PCR%Western blot
目的:探讨FOXO3在肺癌组织与肺癌干细胞中的表达特点,为肺癌的诊断和治疗提供新的着手点。方法应用免疫组化、RT-PCR及Western blot法检测肺癌组织中FOXO3的表达,同时应用RT-PCR和Western blot法验证肺癌干细胞中FOXO3 mRNA及蛋白的表达,比较其在不同分型肺癌中的阴性率。结果免疫组化结果显示FOXO3在肺癌组织中呈明显低表达, RT-PCR和Western blot检测结果显示肺癌组织及肺癌干细胞中FOXO3 mRNA及蛋白均呈明显低表达,FOXO3蛋白在所有病例中的阴性率为66.7%,且随着癌细胞的分化及转移,其阴性率逐渐升高。结论 FOXO3在肺癌组织及肺癌干细胞中均呈低表达,同时其与肺癌的恶性程度、转移密切相关,或许可成为治疗肺癌的新靶点。
目的:探討FOXO3在肺癌組織與肺癌榦細胞中的錶達特點,為肺癌的診斷和治療提供新的著手點。方法應用免疫組化、RT-PCR及Western blot法檢測肺癌組織中FOXO3的錶達,同時應用RT-PCR和Western blot法驗證肺癌榦細胞中FOXO3 mRNA及蛋白的錶達,比較其在不同分型肺癌中的陰性率。結果免疫組化結果顯示FOXO3在肺癌組織中呈明顯低錶達, RT-PCR和Western blot檢測結果顯示肺癌組織及肺癌榦細胞中FOXO3 mRNA及蛋白均呈明顯低錶達,FOXO3蛋白在所有病例中的陰性率為66.7%,且隨著癌細胞的分化及轉移,其陰性率逐漸升高。結論 FOXO3在肺癌組織及肺癌榦細胞中均呈低錶達,同時其與肺癌的噁性程度、轉移密切相關,或許可成為治療肺癌的新靶點。
목적:탐토FOXO3재폐암조직여폐암간세포중적표체특점,위폐암적진단화치료제공신적착수점。방법응용면역조화、RT-PCR급Western blot법검측폐암조직중FOXO3적표체,동시응용RT-PCR화Western blot법험증폐암간세포중FOXO3 mRNA급단백적표체,비교기재불동분형폐암중적음성솔。결과면역조화결과현시FOXO3재폐암조직중정명현저표체, RT-PCR화Western blot검측결과현시폐암조직급폐암간세포중FOXO3 mRNA급단백균정명현저표체,FOXO3단백재소유병례중적음성솔위66.7%,차수착암세포적분화급전이,기음성솔축점승고。결론 FOXO3재폐암조직급폐암간세포중균정저표체,동시기여폐암적악성정도、전이밀절상관,혹허가성위치료폐암적신파점。
Purpose To detect the expression of FOXO3 in lung cancer specimens and lung cancer stem cell, for diagnosis and treat-ment of lung cancer. Methods Immunohistochemistry, semi-quantitative PCR and Western blot were used to detect FOXO3 protein expression in clinical specimens of lung cancer. PCR and Western blot verified FOXO3 mRNA and protein expression of lung cancer cells. Results Immunohistochemistry showed that the FOXO3 was significantly lower expressed in lung cancer tissues and the corre-sponding lung cancer stem cells;Detection of FOXO3 mRNA and protein by RT-PCR and Western blot showed both had significantly lower expression;as progression of the disease, there was a clear decreasing trend, in which negative expression rate of FOXO3 protein accounted for 66. 7% in all patients;at the same times, the rate of the expression gradually decreased with loss of differentiation and metastasis of the tumor. Conclusions FOXO3 shows low expression in lung tissue and lung cancer stem cells, while it is closely relat-ed to the degree of malignancy and lung cancer metastasis, which may be a new target for the treatment.