CAJ | 학술논문

在碱性条件下,头孢曲松钠加热会降解为两种巯基( R’ SH,R″SH)化合物。在pH为4．5时,R’ SH 可使Cu( II)还原为Cu( I),所生成的Cu( I)与R”SH中的巯基生成白色溶胶,导致体系吸光度发生变化。在0．26~160．00μg·mL-1范围内,△A340nm与头孢曲松钠浓度(ρ)之间存在良好的线性关系,回归方程为△A=0．019ρ+0．0079,相关系数为0．9998,检出限0．078μg·mL-1。将本方法直接用于针剂中头孢曲松钠的测定,具有仪器简单、线性范围宽,重现性好的特点。
재감성조건하,두포곡송납가열회강해위량충구기( R’ SH,R″SH)화합물。재pH위4．5시,R’ SH 가사Cu( II)환원위Cu( I),소생성적Cu( I)여R”SH중적구기생성백색용효,도치체계흡광도발생변화。재0．26~160．00μg·mL-1범위내,△A340nm여두포곡송납농도(ρ)지간존재량호적선성관계,회귀방정위△A=0．019ρ+0．0079,상관계수위0．9998,검출한0．078μg·mL-1。장본방법직접용우침제중두포곡송납적측정,구유의기간단、선성범위관,중현성호적특점。
In alkaline conditions,ceftriaxone sodium could be degraded into two kinds of thiol compounds(R'SH,R″SH). In pH 4. 7,R'SH could reduce Cu(II)to Cu(I)and Cu(I)could generate white sol with R″SH,which lead to the change of absorbance in the system. The absorption intensity at 340nm is linear to ceftriaxone sodium concentration in the range of 0. 26~160μg·mL-1 ,The linear equation is△A=0. 019ρ+0. 0079,relation coefficient is 0. 9998 and detection limit is 0. 078μg·mL-1. The proposed method has been applied to determination of ceftriaxone sodium in injection with simplicity of instrument and reagents,good reproducibility and wide linear range.