CAJ | 학술논문

目的：研究姜黄素诱导黑素瘤细胞A375、M14凋亡的机制。方法：将对数生长的黑素瘤细胞A375、M14分别用姜黄素干预；显微镜下观察黑素瘤细胞株A375和M14的凋亡状态；应用Western Blot方法检测凋亡相关蛋白Bcl-2、Bax、Caspase-3、γ-H2AX、NF-κB的变化；应用流式细胞仪检测细胞凋亡率。结果：实验发现姜黄素干预黑素瘤细胞后可见细胞发生凋亡；在姜黄素诱导黑素瘤细胞凋亡过程中，凋亡相关蛋白Bax和γ-H2AX表达水平较对照组升高，而Bcl-2、NF-κB表达水平较较对照组下降，有活性的Caspase-3生成；随着姜黄素的浓度增加，黑色素瘤细胞早期凋亡的比例增加。结论：姜黄素诱导黑素瘤细胞生长凋亡的机制可能与其对凋亡相关蛋白Bcl-2、Bax、Caspase-3、γ-H2AX、NF-κB的表达调控有关。
목적：연구강황소유도흑소류세포A375、M14조망적궤제。방법：장대수생장적흑소류세포A375、M14분별용강황소간예；현미경하관찰흑소류세포주A375화M14적조망상태；응용Western Blot방법검측조망상관단백Bcl-2、Bax、Caspase-3、γ-H2AX、NF-κB적변화；응용류식세포의검측세포조망솔。결과：실험발현강황소간예흑소류세포후가견세포발생조망；재강황소유도흑소류세포조망과정중，조망상관단백Bax화γ-H2AX표체수평교대조조승고，이Bcl-2、NF-κB표체수평교교대조조하강，유활성적Caspase-3생성；수착강황소적농도증가，흑색소류세포조기조망적비례증가。결론：강황소유도흑소류세포생장조망적궤제가능여기대조망상관단백Bcl-2、Bax、Caspase-3、γ-H2AX、NF-κB적표체조공유관。
Objective:To observe the effects of curcumin on the apoptosis of two melanoma cell lines A375 and M14. Methods:Cell apoptosis in human melanoma cell lines A375 and M14 treated with curcumin was measured by morphological method. Furthermore four apoptosis related proteins:Bcl-2, Bax, Caspase-3,γ-H2AX and NF-κB were determined by Western Blotting analysis. Apoptosis rate were detected through FCM (lfow cytometry). Results:hTe data showed that curcumin can induce tumor cell apoptosis. Western blotting analysis conifrmed that curcumin could express increasing proteins Bax andγ-H2AX, down-regulating the proteins Bcl-2, and NF-κB, as well as, activation of Caspase-3. It showed that with the increase of the concentration of curcumin, melanoma cell early apoptosis ratio increased. Conclusion:hTis study showed that curcumin exhibited a remarkably increased apoptosis-inducing effects in melanoma cells.