化学研究与应用
化學研究與應用
화학연구여응용
Chemical Research and Application
2015年
9期
1322-1331
,共10页
盛维娟%刘守信%王芹芹%李璇%光娜儿
盛維娟%劉守信%王芹芹%李璇%光娜兒
성유연%류수신%왕근근%리선%광나인
PEG类聚物%温度敏感性%水凝胶%ATRP%流变学性质%药物释放
PEG類聚物%溫度敏感性%水凝膠%ATRP%流變學性質%藥物釋放
PEG류취물%온도민감성%수응효%ATRP%류변학성질%약물석방
PEG analogues%temperature sensitivity%hydrogel%atom transfer radical polymerization(ATRP)%click reaction%rheologi-cal property%drug release
利用原子转移自由基聚合(ATRP)方法合成了新的温度敏感性聚合物聚(2-甲基-2-丙烯酸-2-(2-甲氧基乙氧基)乙酯-co-寡聚乙二醇甲醚甲基丙烯酸酯-co-N-羟甲基丙烯酰胺)[ P ( MEO2 MA-co-OEGMA-co-HMAM)]。红外光谱( FTIR)、核磁共振谱(1 H NMR)和凝胶渗透色谱( GPC)对其结构进行了表征;利用透光率测定研究了共聚物的温度敏感性以及盐对其低临界溶解温度(LCST)的影响。将共聚物P(MEO2MA-co-OEGMA-co-HMAM)链上的羟基分别转化为叠氮基和炔基后,在抗坏血酸钠和CuSO4的催化条件下,通过温敏性共聚物P( MEO2 MA-co-OEGMA-co-HMAM)链间点击自交联反应获得了温敏性水凝胶。凝胶形成流变动力学研究发现凝胶化作用在1 min之内发生,之后体系的储能模量( G′)大于损耗模量( G″);通过SEM和溶胀性能分析表明,随着温度的升高水凝胶的孔径减小,导致水凝胶的保水量逐渐减小,证明了合成的凝胶具有温度敏感性;牛血清蛋白( BSA)和茴香脑分别作为亲水和疏水药物模型研究凝胶的药物体外持续释放。结果表明:在37 o C时,载药凝胶对BSA释放率较大,释放速度较快。
利用原子轉移自由基聚閤(ATRP)方法閤成瞭新的溫度敏感性聚閤物聚(2-甲基-2-丙烯痠-2-(2-甲氧基乙氧基)乙酯-co-寡聚乙二醇甲醚甲基丙烯痠酯-co-N-羥甲基丙烯酰胺)[ P ( MEO2 MA-co-OEGMA-co-HMAM)]。紅外光譜( FTIR)、覈磁共振譜(1 H NMR)和凝膠滲透色譜( GPC)對其結構進行瞭錶徵;利用透光率測定研究瞭共聚物的溫度敏感性以及鹽對其低臨界溶解溫度(LCST)的影響。將共聚物P(MEO2MA-co-OEGMA-co-HMAM)鏈上的羥基分彆轉化為疊氮基和炔基後,在抗壞血痠鈉和CuSO4的催化條件下,通過溫敏性共聚物P( MEO2 MA-co-OEGMA-co-HMAM)鏈間點擊自交聯反應穫得瞭溫敏性水凝膠。凝膠形成流變動力學研究髮現凝膠化作用在1 min之內髮生,之後體繫的儲能模量( G′)大于損耗模量( G″);通過SEM和溶脹性能分析錶明,隨著溫度的升高水凝膠的孔徑減小,導緻水凝膠的保水量逐漸減小,證明瞭閤成的凝膠具有溫度敏感性;牛血清蛋白( BSA)和茴香腦分彆作為親水和疏水藥物模型研究凝膠的藥物體外持續釋放。結果錶明:在37 o C時,載藥凝膠對BSA釋放率較大,釋放速度較快。
이용원자전이자유기취합(ATRP)방법합성료신적온도민감성취합물취(2-갑기-2-병희산-2-(2-갑양기을양기)을지-co-과취을이순갑미갑기병희산지-co-N-간갑기병희선알)[ P ( MEO2 MA-co-OEGMA-co-HMAM)]。홍외광보( FTIR)、핵자공진보(1 H NMR)화응효삼투색보( GPC)대기결구진행료표정;이용투광솔측정연구료공취물적온도민감성이급염대기저림계용해온도(LCST)적영향。장공취물P(MEO2MA-co-OEGMA-co-HMAM)련상적간기분별전화위첩담기화결기후,재항배혈산납화CuSO4적최화조건하,통과온민성공취물P( MEO2 MA-co-OEGMA-co-HMAM)련간점격자교련반응획득료온민성수응효。응효형성류변동역학연구발현응효화작용재1 min지내발생,지후체계적저능모량( G′)대우손모모량( G″);통과SEM화용창성능분석표명,수착온도적승고수응효적공경감소,도치수응효적보수량축점감소,증명료합성적응효구유온도민감성;우혈청단백( BSA)화회향뇌분별작위친수화소수약물모형연구응효적약물체외지속석방。결과표명:재37 o C시,재약응효대BSA석방솔교대,석방속도교쾌。
A novel temperature responsive copolymer,poly(2-(2-methoxyethoxy)ethyl methacrylate-co-oligo(ethylene glycol)meth-acrylate-co-N-hydroxymethylacrylamide) [ P ( MEO2 MA-co-OEGMA-co-HMAM ) ] , was synthesized by atom transfer radical poly-merization(ATRP). The copolymer was characterized by FTIR,1H NMR,and gel permeation chromatography(GPC). Temperature sensitivity for the copolymer and the effects of salts on the lower critical solution temperature( LCST) of copolymer aqueous solutions were studied by transmittance measurements at different temperatures. Then,the hydroxyl groups on P(MEO2MA-co-OEGMA-co-HMAM) chains were transformed into azide or alkyne groups respectively. A temperature-sensitivity self-cross linking hydrogel was prepared by click chemistry among the copolymers P(MEO2MA-co-OEGMA-co-HMAM). Rheological kinetics of gel formation dem-onstrated that gelation had commenced within 1 minutes,and since then the storage modulus( G′) was higher than the loss modulus (G″). The pore size and swelling ratio of the prepared hydrogels decreased with increasing the temperature because hydrophobic polymer-polymer interactions results in the self-aggregation of PMOH chains at a higher temperature ( T>LCST ) , that proved the formed hydrogel had temperature sensitivities. BSA and anethole respectively were used as a model hydrophilic and hydrophobic drug to examine in vitro the sustained release from hydrogel. Results showed that the anethole release rate from hydrogel within 10 h at 37℃ was slower than BSA.