CAJ | 학술논문

病毒诱导的基因沉默（VIGS）技术是近年发展起来研究植物基因功能的新方法。利用含有目的基因片段的病毒载体侵染植物，导致植物体内相应的功能基因表达受到抑制成为沉默基因，相应功能缺失，从而推测目的基因的功能。该文克隆了烟草番茄红素脱氢酶（PDS ）基因片段，插入到烟草脆裂病毒载体（T RV ）中，构建了pTRV‐PDS的 VIGS载体，转入农杆菌侵染烟草后成功沉默了PDS基因，使烟草叶片出现白化表型，成功建立了烟草VIGS体系。将VIGS这个当前生物学研究热点引入教学实验中，改革更新实验教学内容，有益于学生扩展科研思维。
병독유도적기인침묵（VIGS）기술시근년발전기래연구식물기인공능적신방법。이용함유목적기인편단적병독재체침염식물，도치식물체내상응적공능기인표체수도억제성위침묵기인，상응공능결실，종이추측목적기인적공능。해문극륭료연초번가홍소탈경매（PDS ）기인편단，삽입도연초취렬병독재체（T RV ）중，구건료pTRV‐PDS적 VIGS재체，전입농간균침염연초후성공침묵료PDS기인，사연초협편출현백화표형，성공건립료연초VIGS체계。장VIGS저개당전생물학연구열점인입교학실험중，개혁경신실험교학내용，유익우학생확전과연사유。
VIGS is a recently developed approach for the gene function analysis in plant .Virus vectors with target gene being transformed into the plant can result in the endogenous gene expression silent ,followed with the loss of corresponding function in plant .VIGS technology uses this mechanism to silence plant genes in order to determine their function .Phytoene desaturase (PDS) gene fragment is cloned into tobacco rattle virus vector (TRV) to obtain pTRV‐PDS .The construction is transformed into A grobacterium GV3101 and then infected N .benthamiana .Silencing of the PDS gene in N .benthamiana can result in the white leaf due to photo‐bleaching ,indicating successful establishment of the VIGS system .Introduction of VIGS ,the current hot research ,into the experimental teaching is beneficial to expanding the students’ scientific field .