CAJ | 학술논문

用壳寡糖及酰化壳寡糖与氯化钕反应,合成了壳寡糖-钕和酰化壳寡糖-钕配合物,利用红外光谱( IR)、紫外光谱( UV)手段对其结构进行了表征。在模拟生理条件下,本文采用紫外光谱和荧光光谱研究了两种配合物与牛血清白蛋白( BSA)的相互作用,计算了配合物与BSA的结合常数、结合位点数。荧光光谱结果表明,配合物均可有规律地猝灭BSA的内源荧光,猝灭方式为静态猝灭,壳寡糖-钕和酰化壳寡糖-钕分别与BSA的结合常数为1．33×104L·mol-1和6．95×104L·mol-1,结合位点数为1．05和1．3,说明配合物与BSA均具有较强的结合作用,能够被BSA储存和运输,并且酰化壳寡糖-钕与BSA的结合能力强于壳寡糖-钕。最后采用紫外光谱法对其作用机理进一步确认。因此,酰化壳寡糖-钕可以被BSA存储和运输,有望成为蛋白质荧光探针。
용각과당급선화각과당여록화녀반응,합성료각과당-녀화선화각과당-녀배합물,이용홍외광보( IR)、자외광보( UV)수단대기결구진행료표정。재모의생리조건하,본문채용자외광보화형광광보연구료량충배합물여우혈청백단백( BSA)적상호작용,계산료배합물여BSA적결합상수、결합위점수。형광광보결과표명,배합물균가유규률지졸멸BSA적내원형광,졸멸방식위정태졸멸,각과당-녀화선화각과당-녀분별여BSA적결합상수위1．33×104L·mol-1화6．95×104L·mol-1,결합위점수위1．05화1．3,설명배합물여BSA균구유교강적결합작용,능구피BSA저존화운수,병차선화각과당-녀여BSA적결합능력강우각과당-녀。최후채용자외광보법대기작용궤리진일보학인。인차,선화각과당-녀가이피BSA존저화운수,유망성위단백질형광탐침。
The coordination complexes of chitooligosaccharide with Nd3+( CS-Nd) and succinic-chitooligosaccharide with Nd3+( BCS-Nd)were prepared,which were synthesized by the reaction of chitooligosaccharide and succinic-chitooligosaccharide with NdCl3. The coordination complexes were characterized by infra-red spectrum and ultraviolet absorption spectrum,respectively. Moreover,the in-teraction between complexes and bovine serum albumins( BSA) were studied under simulated physiological conditions by ultraviolet and fluorescence spectrum. Fluorescence experiment studies indicated that the reaction between the complexes and BSA was a static quenching procedure. The binding constant and binding sites are calculated for BSA with CS-Nd were 1. 33×104L·mol-1and1. 05, BSA with BCS-Nd were 6. 95×104L·mol-1and 1. 30,respectively. There was a strong binding reaction between complexes and BSA, and the rare earth complexes could be transported by BSA. The absorption intensity of BSA increased with the increasing of the com-plexes concentration,and shifted to lower wavelength of the maximum absorption peak with a blue. Therefore,BCS-Nd was expected to become a protein fluorescence probe.