医学信息
醫學信息
의학신식
Medical Information
2015年
36期
32-32
,共1页
献血者%核酸检测%隐匿型乙型肝炎%窗口期
獻血者%覈痠檢測%隱匿型乙型肝炎%窗口期
헌혈자%핵산검측%은닉형을형간염%창구기
Blood donors%Nucleic acid detection(NAT)%Occult hepatitis B%Window period
目的 ELISA检测HBsAg结果阴性的无偿献血者样本进行核酸检测，再对核酸检测阳性结果样本进行“两对半”检测并对献血者追踪检测，分析是窗口期感染还是隐匿型乙型肝炎。方法使用双ELISA试剂检测无偿献血者样本，对检测结果阴性的无偿献血者样本使用浩源核酸筛查系统进行核酸检测，对核酸检测阳性结果样本进行“两对半”检测并对献血者追踪检测。结果 HBsAg(ELISA)阴性献血者血液样本12548份进行核酸检测，结果有13份样本检出HBV DNA，通过“两对半”检测以及对献血者追踪检测，确定2例为窗口期感染，11例为隐匿型乙型肝炎。结论核酸检测应用于血液筛查，防止输血传播疾病有及其重要的意义。
目的 ELISA檢測HBsAg結果陰性的無償獻血者樣本進行覈痠檢測，再對覈痠檢測暘性結果樣本進行“兩對半”檢測併對獻血者追蹤檢測，分析是窗口期感染還是隱匿型乙型肝炎。方法使用雙ELISA試劑檢測無償獻血者樣本，對檢測結果陰性的無償獻血者樣本使用浩源覈痠篩查繫統進行覈痠檢測，對覈痠檢測暘性結果樣本進行“兩對半”檢測併對獻血者追蹤檢測。結果 HBsAg(ELISA)陰性獻血者血液樣本12548份進行覈痠檢測，結果有13份樣本檢齣HBV DNA，通過“兩對半”檢測以及對獻血者追蹤檢測，確定2例為窗口期感染，11例為隱匿型乙型肝炎。結論覈痠檢測應用于血液篩查，防止輸血傳播疾病有及其重要的意義。
목적 ELISA검측HBsAg결과음성적무상헌혈자양본진행핵산검측，재대핵산검측양성결과양본진행“량대반”검측병대헌혈자추종검측，분석시창구기감염환시은닉형을형간염。방법사용쌍ELISA시제검측무상헌혈자양본，대검측결과음성적무상헌혈자양본사용호원핵산사사계통진행핵산검측，대핵산검측양성결과양본진행“량대반”검측병대헌혈자추종검측。결과 HBsAg(ELISA)음성헌혈자혈액양본12548빈진행핵산검측，결과유13빈양본검출HBV DNA，통과“량대반”검측이급대헌혈자추종검측，학정2례위창구기감염，11례위은닉형을형간염。결론핵산검측응용우혈액사사，방지수혈전파질병유급기중요적의의。
Objective ELISA in the detection of HBsAg negative blood donation sample nucleic acid detection, and of nucleic acid positive test results for samples of "two half and half" detection and the donors track detection, is to analyze the infection in window period or occult hepatitis B. Methods Double ELISA reagent testing samples of unpaid blood donors, negative unpaid donors samples using the result of the test grandsoo DNA screening system for nucleic acid detection, the nucleic acid testing positive results "two half-and-half" samples and blood donors track detection.Results HBsAg (ELISA) negative blood samples of 12548 blood donors for nucleic acid detection, the results of 13 samples detected HBV DNA, by "two half-and-half" detection and track testing of blood donors and to determine 2 cases for the window period, 11 cases of hepatitis b virus infection aslar-pacific perspective. Conclusion Nucleic acid detection applied in blood screening to prevent blood transfusion transmit ed diseases and its important significance.
