CAJ | 학술논문

目的：探讨白藜芦醇与涎腺腺样囊性癌细胞系ACC-M增殖及凋亡的相关性。方法使用终浓度各为12骀．5、25、50、100和200μmol／L的白藜芦醇处理ACC-M24、48、72 h，并用MTT比色、倒置显微镜观察、光镜观察、流式细胞仪检测并观察ACC-M细胞增殖及凋亡状况。结果白藜芦醇对ACC-M生长成时间-浓度依赖性抑制，最大抑制率可达92．6％。白藜芦醇对ACC-M凋亡有诱导作用，凋亡率与经白藜芦醇作用时间及作用浓度呈正相关（ P ＜0．05）。白藜芦醇可造成ACC-M坏死。结论白藜芦醇通过诱导涎腺腺样囊性癌细胞系ACC-M凋亡及坏死，抑制ACC-M增殖。
목적：탐토백려호순여연선선양낭성암세포계ACC-M증식급조망적상관성。방법사용종농도각위12태．5、25、50、100화200μmol／L적백려호순처리ACC-M24、48、72 h，병용MTT비색、도치현미경관찰、광경관찰、류식세포의검측병관찰ACC-M세포증식급조망상황。결과백려호순대ACC-M생장성시간-농도의뢰성억제，최대억제솔가체92．6％。백려호순대ACC-M조망유유도작용，조망솔여경백려호순작용시간급작용농도정정상관（ P ＜0．05）。백려호순가조성ACC-M배사。결론백려호순통과유도연선선양낭성암세포계ACC-M조망급배사，억제ACC-M증식。
Objective To observe the effect of resveratrol on human salivary adenoid cystic carcinoma cell line-ACC-Min vitro.Methods The ACC-M cells were treated with resveratrol at 12.5,25,50,100, 200μmol/L for 24h, 48h, 72h,respectively.The proliferation and apoptosis of ACC-M cells were observed by MTT assay, inverted microscopy, light microscopy,flow cytometry.Results Resveratrol inhibited the proliferation of ACC-M cells in a dose and time dependent manner.The highest inhibition rate was 92.6%.Resveratrol induced apoptosis and necrosis of ACC-M cells,and the apoptotic rate was positively correlated to the action time and action concentration of resveratrol ( P <0.05).Conclusion Resveratrol can inhibit the proliferation of ACC-M cells by inducing apoptosis and/or necrosis of the cells.