山东农业科学
山東農業科學
산동농업과학
Shandong Agricultural Sciences
2015年
9期
11-13
,共3页
何平%王玉霞%李林光%王海波%李慧峰%孙清荣%程来亮
何平%王玉霞%李林光%王海波%李慧峰%孫清榮%程來亮
하평%왕옥하%리림광%왕해파%리혜봉%손청영%정래량
苹果%三倍性杂种%ISSR%遗传分析
蘋果%三倍性雜種%ISSR%遺傳分析
평과%삼배성잡충%ISSR%유전분석
Apple%Triploid hybrid progeny%ISSR%Genetic analysis
利用 ISSR 分子标记技术对苹果‘寒富’与‘四倍体嘎拉’杂交的三倍性后代及亲本进行遗传变异研究。结果表明9条 ISSR 引物共扩增出多态性条带58条，多态性比率为67．4％。相似性分析可知，三倍性新种质与母体相似系数在0．5909～0．7692之间，与父本相似系数在0．5714～0．7500之间，聚类结果显示三倍性杂交后代具有母本倾向。
利用 ISSR 分子標記技術對蘋果‘寒富’與‘四倍體嘎拉’雜交的三倍性後代及親本進行遺傳變異研究。結果錶明9條 ISSR 引物共擴增齣多態性條帶58條，多態性比率為67．4％。相似性分析可知，三倍性新種質與母體相似繫數在0．5909～0．7692之間，與父本相似繫數在0．5714～0．7500之間，聚類結果顯示三倍性雜交後代具有母本傾嚮。
이용 ISSR 분자표기기술대평과‘한부’여‘사배체알랍’잡교적삼배성후대급친본진행유전변이연구。결과표명9조 ISSR 인물공확증출다태성조대58조，다태성비솔위67．4％。상사성분석가지，삼배성신충질여모체상사계수재0．5909～0．7692지간，여부본상사계수재0．5714～0．7500지간，취류결과현시삼배성잡교후대구유모본경향。
The genetic variation of triploid hybrid progenies from ‘Hanfu’ב4 x Gala’were studied based on ISSR molecular markers in this paper.The results showed that 58 polymorphic bands were amplified by 9 ISSR primers,the percentage of polymorphic bands of ISSR was 67.4%.The genetic similarity was 0.5909 ~0.7692 between triploid hybrid progenies and female parent,and it was 0.5714 ~0.7500 between triploid hybrid progenies and male parent.The cluster result showed that most of triploid hybrid progenies were tend to the female parent ‘Hanfu’.
