CAJ | 학술논문

脂肪酸去饱和酶基因(FAD2)是控制植物体中油酸含量的关键基因,在甘蓝型油菜中有4个FAD2基因的拷贝,分别定位在A1、C1、A5、C5染色体上。本文克隆了1个FAD2拷贝基因,依据油菜基因组数据库信息,将其定位到C1染色体上,命名为BnFAD2-C1,其开放阅读框为1155 bp。采用RACE (rapid-amplification of cDNA ends)技术获得了175 bp的5￠ UTR序列和212 bp的3￠ UTR序列。采用荧光定量PCR技术研究发现,BnFAD2-C1在根、花和角果皮中仅保持本底水平的表达,在种子发育中期呈现高效表达,具有种子特异性诱导表达的特征。根据甘蓝和油菜基因组数据库信息,同源克隆到BnFAD2-C1基因的启动子(promoter)和内含子(intron)序列,并通过PLACE和PlantCARE网站分析,初步预测到调控该基因转录的潜在顺式作用元件。通过茉莉酸诱导处理, BnFAD2-C1基因表达量发生变化,推断茉莉酸在BnFAD2-C1基因的表达过程中可能发挥一定的调控作用。
지방산거포화매기인(FAD2)시공제식물체중유산함량적관건기인,재감람형유채중유4개FAD2기인적고패,분별정위재A1、C1、A5、C5염색체상。본문극륭료1개FAD2고패기인,의거유채기인조수거고신식,장기정위도C1염색체상,명명위BnFAD2-C1,기개방열독광위1155 bp。채용RACE (rapid-amplification of cDNA ends)기술획득료175 bp적5￠ UTR서렬화212 bp적3￠ UTR서렬。채용형광정량PCR기술연구발현,BnFAD2-C1재근、화화각과피중부보지본저수평적표체,재충자발육중기정현고효표체,구유충자특이성유도표체적특정。근거감람화유채기인조수거고신식,동원극륭도BnFAD2-C1기인적계동자(promoter)화내함자(intron)서렬,병통과PLACE화PlantCARE망참분석,초보예측도조공해기인전록적잠재순식작용원건。통과말리산유도처리, BnFAD2-C1기인표체량발생변화,추단말리산재BnFAD2-C1기인적표체과정중가능발휘일정적조공작용。
Fatty acid desaturase gene (FAD2) is a key factor in regulating oleic acid content. There are four copies ofFAD2 lo-cated on chromosomes A1, C1, A5, and C5 inBrassica napus. One copy containing 1155 bp openreading frame was cloned with previous research method and named asBnFAD2-C1, which was location on chromosome C1 based on the genome database in-formation of oleracea and oilseed. The untranslated regions (UTR) of 5′ and 3′ end with 175 bp and 212 bp length respectively were cloned by RACE (rapid-amplification of cDNA ends) technique. The expression pattern ofBnFAD2-C1 gene was identified using quantity PCR technique, showing a seed-specific inducible expression in mid developmental seeds and a background-level expression in root, flower and siliqua wall. The promoter and intron region were also cloned and analyzed using PLACE and PlantCARE websites to predict some potential cis-elements in regulatingBnFAD2-C1gene transcription. At the same time, jas-monic acid (JA) was inferred to make certain contributions to regulateBnFAD2-C1gene expression showing a changeable ex-pression quantity when treated with jasmonic acid.