CAJ | 학술논문

目的：探讨miRNA-143对宫颈癌细胞侵袭能力的影响。方法：采用脂质体转染法瞬时转染miRNA-143过表达和干扰质粒，Transwell迁移实验检测miRNA-143过表达和抑制后宫颈癌细胞侵袭能力的改变，生物信息学预测miRNA-143的作用靶点。miRNA-143过表达和抑制后Western blot及双荧光素酶报告基因检测其靶点MACC1表达，RT-qPCR检测20例患者宫颈癌和癌旁正常组织标本中miRNA-143和MACC1 mRNA的表达，分析20例患者宫颈癌组织中miRNA-143和MACC1 mRNA表达的相关性。结果：Transwell迁移实验显示miRNA-143过表达的宫颈癌细胞的侵袭能力降低，抑制miRNA-143后侵袭能力增强。生物信息学预测显示miRNA-143作用于MACC1的3'-UTR，Western blot及双荧光素酶报告基因结果进一步证实miRNA-143作用于MACC1的3'-UTR。RT-qPCR显示miRNA-143过表达的MACC1 mRNA表达下降，而抑制miRNA-143后MACC1 mRNA表达上升。抑制miRNA-143表达的宫颈癌细胞中MACC1被干扰后，宫颈癌细胞的侵袭能力显著被抑制。宫颈癌组织中miRNA-143表达水平显著低于正常宫颈上皮组织，MACC1表达水平显著高于正常宫颈上皮组织，20例患者的宫颈癌组织中miRNA-143与MACC1 mRNA表达呈负相关。结论：miRNA-143在宫颈癌中表达水平下降，并可能通过靶向MACC1调节宫颈癌细胞的侵袭能力。
목적：탐토miRNA-143대궁경암세포침습능력적영향。방법：채용지질체전염법순시전염miRNA-143과표체화간우질립，Transwell천이실험검측miRNA-143과표체화억제후궁경암세포침습능력적개변，생물신식학예측miRNA-143적작용파점。miRNA-143과표체화억제후Western blot급쌍형광소매보고기인검측기파점MACC1표체，RT-qPCR검측20례환자궁경암화암방정상조직표본중miRNA-143화MACC1 mRNA적표체，분석20례환자궁경암조직중miRNA-143화MACC1 mRNA표체적상관성。결과：Transwell천이실험현시miRNA-143과표체적궁경암세포적침습능력강저，억제miRNA-143후침습능력증강。생물신식학예측현시miRNA-143작용우MACC1적3'-UTR，Western blot급쌍형광소매보고기인결과진일보증실miRNA-143작용우MACC1적3'-UTR。RT-qPCR현시miRNA-143과표체적MACC1 mRNA표체하강，이억제miRNA-143후MACC1 mRNA표체상승。억제miRNA-143표체적궁경암세포중MACC1피간우후，궁경암세포적침습능력현저피억제。궁경암조직중miRNA-143표체수평현저저우정상궁경상피조직，MACC1표체수평현저고우정상궁경상피조직，20례환자적궁경암조직중miRNA-143여MACC1 mRNA표체정부상관。결론：miRNA-143재궁경암중표체수평하강，병가능통과파향MACC1조절궁경암세포적침습능력。
Objective:To illustrate the role of miRNA-143 on the invasiveness of cervical cancer cells. Methods:MiRNA-143 mimics or inhibitor sequences were transiently expressed in the cervical cancer cells by liposome transfection. Transwell assay was ap-plied to test the invasive ability of cervical cancer cells after miRNA-143 over-expression or inhibition. Bioinformatics assay was used to predict the targets of miRNA-143. RT-qPCR and luciferase reporter assay were performed to detect the expression of MACC1 mRNA in the cancer cells. RT-qPCR was conducted to test the expression of miRNA-143 and MACC1 mRNA in 20 fresh primary cervi-cal cancer and their matched para-neoplastic tissues. Statistical analyses were performed to evaluate the association between the expres-sion of miRNA-143 and MACC1 mRNA in the 20 cases of cervical cancer. Results:Transwell assays revealed that the miRNA-143 over-expression inhibited the cell invasiveness, while miRNA-143 inhibition promoted the invasive ability of the cervical cancer cells. Bioinformatics analyses revealed that miRNA-143 could target the 3'-UTR of MACC1. Dual luciferase reporter assay confirmed that miRNA-143 can affect 3'-UTR sequence in MACC1 genes. RT-qPCR analyses indicated that the expression of MACC1 mRNA was ob-viously down-regulated after miRNA-143 over-expression, while significantly increased after the miRNA-143 inhibition. The migration in Caski/miRNA-143 inhibitor cells was obviously elevated after being transfected with MACC1 shRNAs. RT-qPCR analyses showed that the expression of miRNA-143 was obviously decreased in the cancer tissues compared with the normal tissues, while MACC1 mRNA was apparently decreased in cancer tissues compared with the normal ones. Statistical analyses revealed that miRNA-143 was negatively correlated with MACC1 mRNA in the 20 cases of cervical cancer. Conclusion:This study reveals that miRNA-143 is down-regulated in the cervical cancer tissues. MiRNA-143 may play an important role in the regulation of cell invasiveness by targeting MACC1 in the cervical cancer cells.