CAJ | 학술논문

目的：球囊培养富集乳腺癌干性样细胞并检测 hTERT 的表达，进一步通过 RNA 干扰的方法探讨其对乳腺癌细胞自我更新能力的影响。方法球囊培养富集乳腺癌干性样细胞，检测乳腺癌干性细胞与分化细胞中 hTERTmRNA 及蛋白的表达，小分子 RNA 干扰敲低hTERT 的蛋白表达水平。结果乳腺癌干性细胞中 hTERT 表达升高，敲低其表达后乳腺癌细胞自我能力下降。结论高表达的 hTERT 促进了乳腺癌细胞的自我更新能力。
목적：구낭배양부집유선암간성양세포병검측 hTERT 적표체，진일보통과 RNA 간우적방법탐토기대유선암세포자아경신능력적영향。방법구낭배양부집유선암간성양세포，검측유선암간성세포여분화세포중 hTERTmRNA 급단백적표체，소분자 RNA 간우고저hTERT 적단백표체수평。결과유선암간성세포중 hTERT 표체승고，고저기표체후유선암세포자아능력하강。결론고표체적 hTERT 촉진료유선암세포적자아경신능력。
Objective To investigate the expression of hTERT and the effect of hTERT on the self-renewal ability of breast cancer stem cell. Methods Detecting the expression of hTERT by RT- PCR and Western Blot. Knockdown the expression of hTERT by RNA interference. Results We finded out that hTERT is high expressed in breast cancer stem cell, and reduced the expression of hTERT suppressed the self-renewal ability of breast cancer cell. Conclusion High expressed hTERT enhanced the self-renewal ability of breast cancer stem cell.