CAJ | 학술논문

目的观察急性出血坏死性胰腺炎肝损伤中Toll样受体(TLR)2、TLR4 mRNA和蛋白的表达.方法应用逆行胰胆管牛黄胆酸钠注射建造肝损伤动物模型,32只雄性大鼠,假手术组8只,胰腺炎组24只.使用反转录-聚合酶链反应(RT-PCR)方法检测第3、6以及12小时肝脏组织中TLR2以及TLR4 mRNA的表达.结果 3h之后胰腺炎组的大鼠肝组织中的TLR2(0.178E-2±0.931E-4)、TLR4 mRNA (0.171±0.011)开始升高,12 h之后TLR2(0.273E-2±0.834E-4)、TLR4mRNA (0.682±0.014),上升到峰值,假手术组TLR2(0.113E-5±0.156E-6)、TLR4 mRNA(0.112E-2±0.115E-3),比较差异有统计学意义(P＜0.05),且肝脏的损伤程度增加;假手术组大鼠的TLR2和TLR4 mRNA表达变化不明显(P＞0.05).结论急性出血坏死性胰腺炎肝组织中TLR2以及TLR4mRNA基因表达上升很可能在肝脏损伤的发生与发展中起到重要作用.
목적 관찰급성출혈배사성이선염간손상중Toll양수체(TLR)2、TLR4 mRNA화단백적표체.방법 응용역행이담관우황담산납주사건조간손상동물모형,32지웅성대서,가수술조8지,이선염조24지.사용반전록-취합매련반응(RT-PCR)방법검측제3、6이급12소시간장조직중TLR2이급TLR4 mRNA적표체.결과 3h지후이선염조적대서간조직중적TLR2(0.178E-2±0.931E-4)、TLR4 mRNA (0.171±0.011)개시승고,12 h지후TLR2(0.273E-2±0.834E-4)、TLR4mRNA (0.682±0.014),상승도봉치,가수술조TLR2(0.113E-5±0.156E-6)、TLR4 mRNA(0.112E-2±0.115E-3),비교차이유통계학의의(P＜0.05),차간장적손상정도증가;가수술조대서적TLR2화TLR4 mRNA표체변화불명현(P＞0.05).결론 급성출혈배사성이선염간조직중TLR2이급TLR4mRNA기인표체상승흔가능재간장손상적발생여발전중기도중요작용.
Objective To investigate the expression of Toll like receptor (TLR)2,TLR4 mRNA and protein in the liver injury of acute hemorrhagic necrotizing pancreatitis.Methods The animal models of liver injury were established by retrograde bezoar cholic acid sodium injection via cholangiopancreatic duct,and 32 male rats were divided into sham operation group (n =8) and severe acute pancreatitis (SAP) group (n =24).The expression of TLR2 and TLR4 mRNA in liver tissues of different time periods was detected by reverse transcriptase-polymerase chain reaction (RT-PCR).Results In SAP group,the expression of TLR2 (0.178E-2 ± 0.931E-4) and TLR4 mRNA (0.171 ± 0.011) in the liver tissues at 3 h began to increase,and at 12 h,TLR2 (0.273E-2 ±0.834E-4) and TLR4 mRNA (0.682 ±0.014) reached the peak.The peak value of TLR2 mRNA was (0.113E-5 ±0.156E-6),and that of TLR4 mRNA was (0.112E-2 ±0.115E-3) in sham operation group (P ＜ 0.05).The degree of liver injury increased over time.There was no significant change in the expression of TLR2 and TLR4 mRNA in sham operation group.Conclusion The expression of TLR2 and TLR4 mRNA gene in the liver tissue of acute hemorrhagic necrotizing pancreatitis may play an important role in the occurrence and development of liver injury.