中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
9期
2243-2245
,共3页
王昕%黄牛%朱雄兵%谢伟%王国洲%陈双倩%李华驰%马翔%冯茂辉
王昕%黃牛%硃雄兵%謝偉%王國洲%陳雙倩%李華馳%馬翔%馮茂輝
왕흔%황우%주웅병%사위%왕국주%진쌍천%리화치%마상%풍무휘
CD4+ CD25+调节性T细胞%胃癌%白细胞介素-10%流式细胞术%免疫抑制
CD4+ CD25+調節性T細胞%胃癌%白細胞介素-10%流式細胞術%免疫抑製
CD4+ CD25+조절성T세포%위암%백세포개소-10%류식세포술%면역억제
CD4 + CD25 + regulatory T cell%Gastric carcinoma%Interleukin-10%Flow cytometry%Immunosuppression
目的 应用流式细胞术检测胃癌患者外周血中CD4+ CD25+调节性T细胞和血清白细胞介素(IL)-10水平的变化,并探讨其意义.方法 应用免疫荧光流式细胞仪测定65例胃癌患者及35例健康体检者外周血T细胞亚群CD4+ CD25 +/CD4+比值;酶联免疫吸附试验(ELISA)法检测外周血中IL-10的表达水平.结果 胃癌患者外周血CD4+ CD25+/CD4+和CD4 +/CD8+比值分别是(61.12±5.83)％及(6.67±0.47)％,较健康体检者显著增高,两者差异有统计学意义(P＜0.05).胃癌患者及健康体检者外周血IL-10的表达水平分别是(43.41 ±0.68) μg/ml及(21.77±0.54) μg/L,胃癌患者较健康体检者显著增高,两者比较差异有统计学意义(P＜0.05).胃癌患者外周血中CD4+ CD25+T细胞水平与胃癌原发肿瘤的侵润深度、淋巴结转移、临床分期及IL-10呈正相关(P＜0.05).结论 胃癌患者外周血中CD4+ CD25+调节性T细胞增多,胃癌患者免疫功能受到抑制.
目的 應用流式細胞術檢測胃癌患者外週血中CD4+ CD25+調節性T細胞和血清白細胞介素(IL)-10水平的變化,併探討其意義.方法 應用免疫熒光流式細胞儀測定65例胃癌患者及35例健康體檢者外週血T細胞亞群CD4+ CD25 +/CD4+比值;酶聯免疫吸附試驗(ELISA)法檢測外週血中IL-10的錶達水平.結果 胃癌患者外週血CD4+ CD25+/CD4+和CD4 +/CD8+比值分彆是(61.12±5.83)％及(6.67±0.47)％,較健康體檢者顯著增高,兩者差異有統計學意義(P＜0.05).胃癌患者及健康體檢者外週血IL-10的錶達水平分彆是(43.41 ±0.68) μg/ml及(21.77±0.54) μg/L,胃癌患者較健康體檢者顯著增高,兩者比較差異有統計學意義(P＜0.05).胃癌患者外週血中CD4+ CD25+T細胞水平與胃癌原髮腫瘤的侵潤深度、淋巴結轉移、臨床分期及IL-10呈正相關(P＜0.05).結論 胃癌患者外週血中CD4+ CD25+調節性T細胞增多,胃癌患者免疫功能受到抑製.
목적 응용류식세포술검측위암환자외주혈중CD4+ CD25+조절성T세포화혈청백세포개소(IL)-10수평적변화,병탐토기의의.방법 응용면역형광류식세포의측정65례위암환자급35례건강체검자외주혈T세포아군CD4+ CD25 +/CD4+비치;매련면역흡부시험(ELISA)법검측외주혈중IL-10적표체수평.결과 위암환자외주혈CD4+ CD25+/CD4+화CD4 +/CD8+비치분별시(61.12±5.83)％급(6.67±0.47)％,교건강체검자현저증고,량자차이유통계학의의(P＜0.05).위암환자급건강체검자외주혈IL-10적표체수평분별시(43.41 ±0.68) μg/ml급(21.77±0.54) μg/L,위암환자교건강체검자현저증고,량자비교차이유통계학의의(P＜0.05).위암환자외주혈중CD4+ CD25+T세포수평여위암원발종류적침윤심도、림파결전이、림상분기급IL-10정정상관(P＜0.05).결론 위암환자외주혈중CD4+ CD25+조절성T세포증다,위암환자면역공능수도억제.
Objective To evaluate the changes of CD4 + CD25 + T cells in peripheral blood of gastric cancer patients,and observe the relationship between CD4 + CD25 + regulatory T cells and the clinicopathologic features.Methods Sixty-five patients with gastric cancer and 35 health examination volunteers were included in this study.The proportion of CD4 + CD25 + T cells population in total T cells was evaluated by flow cytometry.The cytokine production [interleukin (IL)-10] was measured by enzyme linked immunosorbent assay (ELISA).Results The population of CD4 + CD25 + T cells in peripheral blood from gastric carcinoma patients was (61.12 ± 5.83)％.It is significantly higher than that in health examination volunteers (P ＜0.05).The population of cytokine production (IL-10) in peripheral blood from gastric carcinoma patients was (43.41 ±0.68) μg/L.It is significantly higher than that in health examination volunteers (P ＜ 0.05).The CD4 + CD25 + T cells population in gastric cancer patients was positively correlated with the deepness of invasion and IL-10 level (P ＜ 0.05).Conclusion CD4 + CD25 + regulatory T cell is prevalent in gastric cancer.It may be responsible for immune suppression in gastric cancer patients.
