CAJ | 학술논문

目的：探索海洋来源的拟诺卡菌（Nocardiopsis sp ．） SCSIO 11492的抗肿瘤活性次生代谢产物。方法采用硅胶、凝胶、ODS等色谱分离方法对海洋来源的拟诺卡菌SCSIO 11492的发酵粗提物进行分离、纯化，根据次生代谢产物的核磁数据及相关的理化性质确定化合物的结构，最后利用活性测试发现活性成分。结果从海洋拟诺卡菌SCSIO 11492中分离纯化得到5个化合物，经鉴定分别为2′-脱氧腺苷（2′-deoxyadenosine ，1）、2′-脱氧胸腺嘧啶核苷（2′-deoxythymidine ，2）、2′-脱氧尿苷（2′-deoxyuidine ，3）、尿嘧啶核苷（uridine ，4）、1-O-棕榈酰基-3-O-β-D-半乳糖基甘油酯（1-O-hexadecanoyl-3-O-β-D-ga-lactopyranosylglycerol ，5）。活性测试结果表明化合物5对RAW264．7显示出较好的生长抑制活性（IC50＝10．9μmol／L）。结论从海洋拟诺卡菌SCSIO 11492中分离得到5个化合物，均为首次从该属中分离得到。其中化合物5可能是该菌抗肿瘤的活性成分。
목적：탐색해양래원적의낙잡균（Nocardiopsis sp ．） SCSIO 11492적항종류활성차생대사산물。방법채용규효、응효、ODS등색보분리방법대해양래원적의낙잡균SCSIO 11492적발효조제물진행분리、순화，근거차생대사산물적핵자수거급상관적이화성질학정화합물적결구，최후이용활성측시발현활성성분。결과종해양의낙잡균SCSIO 11492중분리순화득도5개화합물，경감정분별위2′-탈양선감（2′-deoxyadenosine ，1）、2′-탈양흉선밀정핵감（2′-deoxythymidine ，2）、2′-탈양뇨감（2′-deoxyuidine ，3）、뇨밀정핵감（uridine ，4）、1-O-종려선기-3-O-β-D-반유당기감유지（1-O-hexadecanoyl-3-O-β-D-ga-lactopyranosylglycerol ，5）。활성측시결과표명화합물5대RAW264．7현시출교호적생장억제활성（IC50＝10．9μmol／L）。결론종해양의낙잡균SCSIO 11492중분리득도5개화합물，균위수차종해속중분리득도。기중화합물5가능시해균항종류적활성성분。
Objective To explore cytotoxic secondary metabolites from a marine actinomycete Nocardiopsis sp .SCSIO 11492 .Methods Isolation and purification were carried out by column chromatography over silica gel ,Sephadex LH-20 ,and ODS structures of the isolates were identified mainly by NMR spectroscopic data .And cytotoxic bioassay was performed using MTT method .Results Five compounds were identified as 2′-deoxyadenosine (1) ,2′-deoxythymidine (2) ,2′-deoxyuidine (3) ,uridine (4) ,1-O-palmitoyl-3-d-galactosyl-sn-glycerol spongilipid (5) .Compound 5 exhibited weak cytotoxic activity with IC50 value of 10 .9 μmol/L .Conclusion Five compounds were obtained from a marine actinomycete Nocardiopsis sp .SCSIO 11492 .All five compounds were reported for the first time from this genus .Compound 5 could be the bioactive compound re-sponsible for the cytotoxic activity of Nocardiopsis sp .SCSIO 11492 .