CAJ | 학술논문

目的：探讨槐杞黄颗粒单独应用或与泼尼松联用对阿霉素肾病大鼠细胞免疫及体液免疫功能的影响以及对蛋白尿的作用。方法雄性SD大鼠50只,随机平均分为5组(n=10)。4组经尾静脉注射盐酸多柔比星(6．5 mg/kg)制作肾病模型,另一组注射生理盐水为正常对照组。2周后造模成功,每日灌胃治疗6周。给药剂量如下：正常对照组(A组)及肾病生理盐水组( B组)给予生理盐水2 ml；肾病泼尼松组( C组)给予泼尼松液2 mg/kg；肾病槐杞黄组( D组)给予槐杞黄颗粒液2 g/kg；肾病槐杞黄泼尼松联用组( E组)给予槐杞黄液2 g/kg 及泼尼松液2 mg/kg。比较各组死亡率、体重变化趋势、24 h尿蛋白增长量,血清IgA、 IgG、 IgM,以及淋巴细胞亚群CD3+T细胞、 CD3+CD4+T细胞、CD3+CD8+T细胞、 CD45RA+B细胞、 CD161a+NK细胞比率的差异。结果所有大鼠均成功造模,24 h尿蛋白均值为(0．389±0．273) g/24 h。 C、 E组死亡率显著高于A、 B组(P<0．05), C、 D、 E 3组之间差异无统计学意义。正常对照组体重持续增长,其余4组给药2周前体重呈增长趋势,之后呈下降趋势,4组之间差异无统计学意义。4组肾病大鼠给药6周后24 h尿蛋白量均增长,增长量分别为(0．313±0．266)、(0．404±0．235)、(0．120±0．628)、(0．480±0．229) g/24 h,差异无统计学意义。5组之间CD3+T细胞、 CD3+CD4+T细胞、 CD3+CD8+ T细胞、 CD45RA+B细胞、 CD161a+NK细胞在全血中的比率差异均无统计学意义。 B组的IgA、 IgG及IgM显著高于A组； C组的IgA、 IgG及IgM均显著低于B组； D组IgA显著低于B组, IgG显著低于A、 B组, IgM显著高于A、 C组； E组IgA、 IgG显著高于A、 C、 D组, IgM显著高于A、 C组(P均<0．05)；各指标其他两组间比较差异均无统计学意义。结论极严重肾病大鼠体液免疫功能亢进,槐杞黄可发挥双向免疫调节作用,单用时可下调亢进的免疫功能,与泼尼松效果相近；与泼尼松联用时,可上调泼尼松对机体的免疫抑制作用,与泼尼松相拮抗。本研究未发现各种治疗对细胞免疫的影响及对尿蛋白增长量的作用。目的：探討槐杞黃顆粒單獨應用或與潑尼鬆聯用對阿黴素腎病大鼠細胞免疫及體液免疫功能的影響以及對蛋白尿的作用。方法雄性SD大鼠50隻,隨機平均分為5組(n=10)。4組經尾靜脈註射鹽痠多柔比星(6．5 mg/kg)製作腎病模型,另一組註射生理鹽水為正常對照組。2週後造模成功,每日灌胃治療6週。給藥劑量如下：正常對照組(A組)及腎病生理鹽水組( B組)給予生理鹽水2 ml；腎病潑尼鬆組( C組)給予潑尼鬆液2 mg/kg；腎病槐杞黃組( D組)給予槐杞黃顆粒液2 g/kg；腎病槐杞黃潑尼鬆聯用組( E組)給予槐杞黃液2 g/kg 及潑尼鬆液2 mg/kg。比較各組死亡率、體重變化趨勢、24 h尿蛋白增長量,血清IgA、 IgG、 IgM,以及淋巴細胞亞群CD3+T細胞、 CD3+CD4+T細胞、CD3+CD8+T細胞、 CD45RA+B細胞、 CD161a+NK細胞比率的差異。結果所有大鼠均成功造模,24 h尿蛋白均值為(0．389±0．273) g/24 h。 C、 E組死亡率顯著高于A、 B組(P<0．05), C、 D、 E 3組之間差異無統計學意義。正常對照組體重持續增長,其餘4組給藥2週前體重呈增長趨勢,之後呈下降趨勢,4組之間差異無統計學意義。4組腎病大鼠給藥6週後24 h尿蛋白量均增長,增長量分彆為(0．313±0．266)、(0．404±0．235)、(0．120±0．628)、(0．480±0．229) g/24 h,差異無統計學意義。5組之間CD3+T細胞、 CD3+CD4+T細胞、 CD3+CD8+ T細胞、 CD45RA+B細胞、 CD161a+NK細胞在全血中的比率差異均無統計學意義。 B組的IgA、 IgG及IgM顯著高于A組； C組的IgA、 IgG及IgM均顯著低于B組； D組IgA顯著低于B組, IgG顯著低于A、 B組, IgM顯著高于A、 C組； E組IgA、 IgG顯著高于A、 C、 D組, IgM顯著高于A、 C組(P均<0．05)；各指標其他兩組間比較差異均無統計學意義。結論極嚴重腎病大鼠體液免疫功能亢進,槐杞黃可髮揮雙嚮免疫調節作用,單用時可下調亢進的免疫功能,與潑尼鬆效果相近；與潑尼鬆聯用時,可上調潑尼鬆對機體的免疫抑製作用,與潑尼鬆相拮抗。本研究未髮現各種治療對細胞免疫的影響及對尿蛋白增長量的作用。
목적：탐토괴기황과립단독응용혹여발니송련용대아매소신병대서세포면역급체액면역공능적영향이급대단백뇨적작용。방법웅성SD대서50지,수궤평균분위5조(n=10)。4조경미정맥주사염산다유비성(6．5 mg/kg)제작신병모형,령일조주사생리염수위정상대조조。2주후조모성공,매일관위치료6주。급약제량여하：정상대조조(A조)급신병생리염수조( B조)급여생리염수2 ml；신병발니송조( C조)급여발니송액2 mg/kg；신병괴기황조( D조)급여괴기황과립액2 g/kg；신병괴기황발니송련용조( E조)급여괴기황액2 g/kg 급발니송액2 mg/kg。비교각조사망솔、체중변화추세、24 h뇨단백증장량,혈청IgA、 IgG、 IgM,이급림파세포아군CD3+T세포、 CD3+CD4+T세포、CD3+CD8+T세포、 CD45RA+B세포、 CD161a+NK세포비솔적차이。결과소유대서균성공조모,24 h뇨단백균치위(0．389±0．273) g/24 h。 C、 E조사망솔현저고우A、 B조(P<0．05), C、 D、 E 3조지간차이무통계학의의。정상대조조체중지속증장,기여4조급약2주전체중정증장추세,지후정하강추세,4조지간차이무통계학의의。4조신병대서급약6주후24 h뇨단백량균증장,증장량분별위(0．313±0．266)、(0．404±0．235)、(0．120±0．628)、(0．480±0．229) g/24 h,차이무통계학의의。5조지간CD3+T세포、 CD3+CD4+T세포、 CD3+CD8+ T세포、 CD45RA+B세포、 CD161a+NK세포재전혈중적비솔차이균무통계학의의。 B조적IgA、 IgG급IgM현저고우A조； C조적IgA、 IgG급IgM균현저저우B조； D조IgA현저저우B조, IgG현저저우A、 B조, IgM현저고우A、 C조； E조IgA、 IgG현저고우A、 C、 D조, IgM현저고우A、 C조(P균<0．05)；각지표기타량조간비교차이균무통계학의의。결론겁엄중신병대서체액면역공능항진,괴기황가발휘쌍향면역조절작용,단용시가하조항진적면역공능,여발니송효과상근；여발니송련용시,가상조발니송대궤체적면역억제작용,여발니송상길항。본연구미발현각충치료대세포면역적영향급대뇨단백증장량적작용。
Objective To investigate the effects of Huai Qi Huang granules administered alone or com-bined with predisone on humoral immunity, cellular immunity, and proteinuria of adriamycin nephrosis rats. Methods A total of 50 SD rats were randomly divided into 5 groups ( all n=10 ) , in which 4 groups were injec-ted with a single dose of 6. 5 mg/kg adriamycin into the caudal vein to establish nephrosis model, while the other group was injected with normal saline ( NS) as normal control. Two weeks later, the nephrosis rat model was es-tablished successfully. The rats were treated by daily intragastric administration for 6 weeks: the normal control group ( group A) and the nephrosis+NS group ( group B) were treated with 2 ml NS; group C ( nephrosis+predisone group), D (nephrosis +Huai Qi Huang group), and E (nephrosis +predisone +Huai Qi Huang group) were treated with 2 mg/kg prednisone, 2 g/kg Huai Qi Huang granule solution, and 2 mg/kg predni-sone plus 2 g/kg Huai Qi Huang granule solution, respectively. The 5 groups were compared in terms of death rate, weight changes trend, 24-hour urinary protein increment, serum IgA, IgG and IgM, and percentages of lymphocyte subsets of CD3 +T cells, CD3 +CD4 +T cells, CD3 +CD8 +T cells, CD45RA+B cells, CD161a+NK cells. Results All nephrosis rats model were successfully made, with 24-hour urinary protein being (0. 389 ± 0. 273) g/24 h. The death rates of group C and group E were significantly higher than those of group A and group B (P<0. 05), while no significant difference was found among group C, group D, and group E. The body weight of group A was sustainably growing;in contrast, the body weight of the other 4 groups grew in the 2 weeks before administration of treatment, but dropped thereafter, with no significant difference among the 4 groups. Six weeks after administration, 24-hour urinary protein of the 4 nephrosis groups were all increased, by ( 0. 313 ± 0. 266), (0. 404 ± 0. 235), (0. 120 ± 0. 628), and (0. 480 ± 0. 229) g/24 h, respectively, with no signficant difference in increment. There was no significant difference in the percentages of lymphocyte subsets ( CD3 +T cells, CD3 +CD4 +T cells, CD3 +CD8 +T cells, CD45RA+B cells, CD161a+NK cells) among the 5 groups. IgA, IgG, and IgM were higher in group B than in group A;IgA, IgG, and IgM in group C were lower than in group B;in group D, IgA was lower than in group B, IgG lower than in group A and group B, IgM higher than in group A and group C;and in group E, IgA and IgG were higher than in group A, group C and group D, IgM higher than in group A and group C;the differences were all of statistical significance ( all P<0. 05 ) . In addi-tion, there was no significant difference of IgA, IgG, or IgM in the comparison between every other two groups. Conclusions Humoral immunity in severe nephrosis rats is hyperfunctional. Huai Qi Huang is a bidirectional immune modulator. It could down-regulate hyperactive humoral immunity when used alone, similar to the effect of prednisone. When combined with prednisone, it could antagonize the immunosuppression effect of prednisone on the body. No regulation effect of different treatments on cellular immunity or proteinuria was found in this study.