CAJ | 학술논문

目的：：探讨不同培养基对湖南汝城温泉微生物中可培养细菌的多样性.方法：用DSM88、牛肉膏蛋白胨、高氏一号三种培养基分别于50℃培养罗泉温泉和60℃培养热水温泉样品.用稀释涂布平板法和平板划线法分离、纯化嗜热菌并进行革兰染色和生理生化试验。结果：81个菌株中革兰氏染色阳性菌50株,阴性菌31株. DSM88、牛肉膏蛋白胨和高氏一号培养基中罗泉和热水样品分别获得的菌株数为23、15、3,25、6、9.结论：三种培养基中DSM88培养的嗜热菌种类最多；采用不同培养基丰富了可培养嗜热菌的资源.
목적：：탐토불동배양기대호남여성온천미생물중가배양세균적다양성.방법：용DSM88、우육고단백동、고씨일호삼충배양기분별우50℃배양라천온천화60℃배양열수온천양품.용희석도포평판법화평판화선법분리、순화기열균병진행혁란염색화생리생화시험。결과：81개균주중혁란씨염색양성균50주,음성균31주. DSM88、우육고단백동화고씨일호배양기중라천화열수양품분별획득적균주수위23、15、3,25、6、9.결론：삼충배양기중DSM88배양적기열균충류최다；채용불동배양기봉부료가배양기열균적자원.
The paper discusses Rucheng hot - spring microorganism educable bacteria diversity on different medium. Using three kinds of medium to cultivate hot spring microorganism, by way of DSM88 medium, Beef extract peptone medium and Gao's medium to cultivate of hot springs microbial, Warmspring to 50℃,hotspring to 60 ℃. By attenuating coating method and plating streak method, separating and purifying hot-spring thermophilic bacteria and makes experiment of Gram's staining and physiological - biochemical test. Moreover, do morphological observation and physic - chemical property experiment. All the isolated strains were gram positive. Separated twenty - three kinds of bacteria from warm - spring by DSM88 medium, five strains of staphylococcus aureus and eighteen of pole state; one strains of staphylococcus aureus and fourteen of pole state from beef extract peptone medium;three kinds of rod -shaped bacteria from Gao's medium. Twenty -five kinds of bacteria from hot spring with DSM88 medium and all are rod - shaped bacteria; six rod - shaped bacteria from beef extract peptone medium and nine kinds of gram positive rod - shaped bacteria from Gao's medium. DSM88 medium is a kind of normal medium using for hot-spring thermophilic bacteria, it can get more educable thermophilic bacteria resource with different mediums.