CAJ | 학술논문

目的：研究 RNA 干扰对宫颈鳞癌 SiHa 细胞中 HIF-1α及survivin 的抑制效应。方法使用不同缺氧时间分别缺氧 SiHa 细胞，以Westernblot 法检测细胞中 HIF-1a 蛋白的表达变化，选择可以引起HIF-1a 蛋白表达增加的缺氧培养时间。进行缺氧后用 Westernblot 法和逆转录聚合酶链反应检测 HIF-1α及 survivin 的表达。结果 SiHa细胞 HIF-1α的表达在缺氧处理时较常氧培养时增加，在缺氧后24～48小时内明显升高，60小时略有降低，72～96 h 逐渐减少。氯化钴培养后 SiHa 细胞的 HIF-1a 蛋白及 survivin 蛋白表达增加，并与氯化钴浓度有关。通过 RNA 干扰技术抑制 HIF-1a 基因的干扰后的细胞中 HIF-1a 及 survivin 表达同时减少。结论在 SiHa 细胞中，HIF-1α基因和 survivin 表达具有高度的相关性，并且抑制 HIF-1α表达可能通过抑制 survivin 而促进癌细胞的凋亡。
목적：연구 RNA 간우대궁경린암 SiHa 세포중 HIF-1α급survivin 적억제효응。방법사용불동결양시간분별결양 SiHa 세포，이Westernblot 법검측세포중 HIF-1a 단백적표체변화，선택가이인기HIF-1a 단백표체증가적결양배양시간。진행결양후용 Westernblot 법화역전록취합매련반응검측 HIF-1α급 survivin 적표체。결과 SiHa세포 HIF-1α적표체재결양처리시교상양배양시증가，재결양후24～48소시내명현승고，60소시략유강저，72～96 h 축점감소。록화고배양후 SiHa 세포적 HIF-1a 단백급 survivin 단백표체증가，병여록화고농도유관。통과 RNA 간우기술억제 HIF-1a 기인적간우후적세포중 HIF-1a 급 survivin 표체동시감소。결론재 SiHa 세포중，HIF-1α기인화 survivin 표체구유고도적상관성，병차억제 HIF-1α표체가능통과억제 survivin 이촉진암세포적조망。
Objective To explore the inhibition effect of RNA interference to HIF-1 alpha and survivin in cervical squamous carcinoma SiHa cells. Methods Deprivate oxygen to SiHa cells in different time, then detecte the changing of HIF-1a protein by Westernblot and choosing the right time which can increase the expression of HIF-1a. Use Westernblot and RT-PCR to detect the expression of HIF - 1 alpha and survivin. Results The HIF-1a expression in SiHa cells was increased by oxygen deprivation, and rised in 24~48 h, decreased a little in 60 h and much more in 72~96 h. The expression of HIF-1a and survivin is related to the concentration of CoCl2, and was decreased by using RNA interference. Conclusion In SiHa cells, the expression of HIF-1a is highly related to survivin so that suppression of HIF-1a could promote apoptosis of cancer cells.