中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
9期
2231-2233
,共3页
核因子-κB p65%皮肤鳞状细胞癌%免疫组织化学
覈因子-κB p65%皮膚鱗狀細胞癌%免疫組織化學
핵인자-κB p65%피부린상세포암%면역조직화학
Nuclear transcription factor-KB p65%Squamous cell carcinoma%Immunohistochemical methods
目的 探讨核因子-κB(NF-κB) p65在皮肤鳞状细胞癌及正常皮肤组织中的表达及意义.方法 采用反转录-聚合酶链反应(RT-PCR)和免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)法,检测40例鳞状细胞癌及40例正常皮肤组织中NF-κB p65 mRNA和蛋白的表达.结果 皮肤鳞状细胞癌组织中NF-κB p65 mRNA相对表达量(6.28±4.01)高于正常皮肤组织中的表达(1.62±0.99),组间比较差异有统计学意义(P＜0.05);免疫组织化学结果显示皮肤鳞状细胞癌组织中NF-κB p65阳性表达率(58.7％)高于正常皮肤组织(11.8％),差异有统计学意义(x2=11.005,P＜0.01).结论 NF-κB p65蛋白可能参与皮肤鳞状细胞癌的形成,减少NF-κB p65蛋白表达的靶向治疗.
目的 探討覈因子-κB(NF-κB) p65在皮膚鱗狀細胞癌及正常皮膚組織中的錶達及意義.方法 採用反轉錄-聚閤酶鏈反應(RT-PCR)和免疫組織化學鏈黴菌抗生物素蛋白-過氧化物酶(SP)法,檢測40例鱗狀細胞癌及40例正常皮膚組織中NF-κB p65 mRNA和蛋白的錶達.結果 皮膚鱗狀細胞癌組織中NF-κB p65 mRNA相對錶達量(6.28±4.01)高于正常皮膚組織中的錶達(1.62±0.99),組間比較差異有統計學意義(P＜0.05);免疫組織化學結果顯示皮膚鱗狀細胞癌組織中NF-κB p65暘性錶達率(58.7％)高于正常皮膚組織(11.8％),差異有統計學意義(x2=11.005,P＜0.01).結論 NF-κB p65蛋白可能參與皮膚鱗狀細胞癌的形成,減少NF-κB p65蛋白錶達的靶嚮治療.
목적 탐토핵인자-κB(NF-κB) p65재피부린상세포암급정상피부조직중적표체급의의.방법 채용반전록-취합매련반응(RT-PCR)화면역조직화학련매균항생물소단백-과양화물매(SP)법,검측40례린상세포암급40례정상피부조직중NF-κB p65 mRNA화단백적표체.결과 피부린상세포암조직중NF-κB p65 mRNA상대표체량(6.28±4.01)고우정상피부조직중적표체(1.62±0.99),조간비교차이유통계학의의(P＜0.05);면역조직화학결과현시피부린상세포암조직중NF-κB p65양성표체솔(58.7％)고우정상피부조직(11.8％),차이유통계학의의(x2=11.005,P＜0.01).결론 NF-κB p65단백가능삼여피부린상세포암적형성,감소NF-κB p65단백표체적파향치료.
Objective To determine whether nuclear transcription factor-κB (NF-κB) p65 expression was altered in skin squamous cell carcinoma (SCC) compared with normal skin.Methods We examined NF-κB p65 expression in 40 cases of SCC and compared to the normal skin (40 cases) by streptavid-in-peroxidase (SP) immunohistochemical methods and real-time quantitative polymerase chain reaction (Real-time PCR).Results The relative content of NF-κB p65 mRNA in SCC tissues (6.28 ± 4.01) was obviously higher than that (1.62 ±0.99) in normal skin tissues (P ＜0.05).The positive ratio of NF-κB p65 protein in SCC tissues was evidently higher than that in normal skin tissues (x2 =11.005,P ＜ 0.01).Conclusion NF-κB p65 protein might be involved in formation of SCC.Target treatment of decreasing the expression of NF-κB p65 might be possible to prevent the formation of SCC.
