CAJ | 학술논문

目的分析肺腺癌原发病灶、癌旁组织、癌症脑转移病灶的基因表达.方法收集1例患者肺腺癌原发病灶、癌旁组织、癌症脑转移病灶的组织样本进行测序,DEGseq算法筛选出差异表达基因.结果与癌旁组织比较,原发病灶中存在着845个基因表达失调(9个上调,836个下调,P＜0.01);癌症脑转移病灶中存在着813个基因表达失调(229个上调,584个下调,P＜0.01);与肺腺癌原发病灶比较,癌症脑转移病灶中存在着218个基因表达失调(130个上调,88个下调,P＜0.01).结论转录组测序可以同步、全面的获取组织样本中的基因表达信息;随着肺腺癌病灶发生脑部转移,差异基因呈表达上调的趋势.
목적 분석폐선암원발병조、암방조직、암증뇌전이병조적기인표체.방법 수집1례환자폐선암원발병조、암방조직、암증뇌전이병조적조직양본진행측서,DEGseq산법사선출차이표체기인.결과 여암방조직비교,원발병조중존재착845개기인표체실조(9개상조,836개하조,P＜0.01);암증뇌전이병조중존재착813개기인표체실조(229개상조,584개하조,P＜0.01);여폐선암원발병조비교,암증뇌전이병조중존재착218개기인표체실조(130개상조,88개하조,P＜0.01).결론 전록조측서가이동보、전면적획취조직양본중적기인표체신식;수착폐선암병조발생뇌부전이,차이기인정표체상조적추세.
Objective To analyze the differentially expressed genes (DEGs) among primary lung cancer,adjacent normal lung tissues and the metastatic brain tissues.Methods The primary lung cancer tissues,adjacent normal lung tissues and metastatic brain tissues were collected from one patient for RNA-Seq analysis.DEGseq was utilized to identify the DEGs.Results Compared with the normal lung tissues,there were 845 DEGs in primary lung cancer tissues (9 up-regulated and 836 down-regulated genes,P ＜ 0.01);there were 813 DEGs in metastatic brain tissues (229 up-regulated and 584 down-regulated genes,P ＜ 0.01);compared with primary lung cancer tissues,there were 218 DEGs in metastatic brain tissues (130 up-regulated and 88 down-regulated genes,P ＜ 0.01).Conclusion RNA-Seq analysis is an effective approach for obtaining the gene expression profile of tissue samples;the DEGs showed an up-regulation trend along with the occurrence of primary lung cancer metastasis to the brain.