中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
9期
2246-2248
,共3页
缺氧诱导因子-1α%血管内皮生长因子%胃癌
缺氧誘導因子-1α%血管內皮生長因子%胃癌
결양유도인자-1α%혈관내피생장인자%위암
Hypoxia inducible factor-1 α%Vascular endothelial growth factor%Gastric carcinoma
目的 探讨缺氧诱导因子-1α(HIF-1 α)和血管内皮生长因子(VEGF)在胃癌组织中的表达及其与肿瘤细胞增殖的关系.方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)法检测58例胃癌组织和癌旁组织中HIF-1α和VEGF的表达,并探讨它们与胃癌临床病理特征的关系,同时用流式细胞仪检测S期细胞比例(SPF).结果 HIF-1α在胃癌组织中阳性表达率为62.07％ (36/58),在癌旁组织中阳性表达率为18.97％(11/58),两者差异有统计学意义(x2=6.49,P＜0.05);淋巴结转移阳性组的阳性表达率为71.88％ (23/32),淋巴结转移阴性组的阳性表达率为26.92(7/26,x2 =6.21,P＜0.05).VEGF在胃癌组织中阳性表达率为65.52％(38/58),在癌旁组织中阳性表达率为22.41％ (13/58),两者差异有统计学意义(x2=5.32,P＜0.05);淋巴结转移阳性组的阳性表达率为68.75％ (22/32),淋巴结转移阴性组的阳性表达率为30.77(8/26,x2=5.43,P＜0.05).胃癌组织中HIF-1α和VEGF的表达与SPF值明显相关;胃癌组织中HIF-1 α和VEGF的表达呈正相关.结论 胃癌组织中HIF-1α和VEGF的表达升高,且两者的表达均与淋巴结转移相关;HIF-1α和VEGF可以促进胃癌肿瘤细胞的增殖.
目的 探討缺氧誘導因子-1α(HIF-1 α)和血管內皮生長因子(VEGF)在胃癌組織中的錶達及其與腫瘤細胞增殖的關繫.方法 採用免疫組織化學鏈黴菌抗生物素蛋白-過氧化物酶(SP)法檢測58例胃癌組織和癌徬組織中HIF-1α和VEGF的錶達,併探討它們與胃癌臨床病理特徵的關繫,同時用流式細胞儀檢測S期細胞比例(SPF).結果 HIF-1α在胃癌組織中暘性錶達率為62.07％ (36/58),在癌徬組織中暘性錶達率為18.97％(11/58),兩者差異有統計學意義(x2=6.49,P＜0.05);淋巴結轉移暘性組的暘性錶達率為71.88％ (23/32),淋巴結轉移陰性組的暘性錶達率為26.92(7/26,x2 =6.21,P＜0.05).VEGF在胃癌組織中暘性錶達率為65.52％(38/58),在癌徬組織中暘性錶達率為22.41％ (13/58),兩者差異有統計學意義(x2=5.32,P＜0.05);淋巴結轉移暘性組的暘性錶達率為68.75％ (22/32),淋巴結轉移陰性組的暘性錶達率為30.77(8/26,x2=5.43,P＜0.05).胃癌組織中HIF-1α和VEGF的錶達與SPF值明顯相關;胃癌組織中HIF-1 α和VEGF的錶達呈正相關.結論 胃癌組織中HIF-1α和VEGF的錶達升高,且兩者的錶達均與淋巴結轉移相關;HIF-1α和VEGF可以促進胃癌腫瘤細胞的增殖.
목적 탐토결양유도인자-1α(HIF-1 α)화혈관내피생장인자(VEGF)재위암조직중적표체급기여종류세포증식적관계.방법 채용면역조직화학련매균항생물소단백-과양화물매(SP)법검측58례위암조직화암방조직중HIF-1α화VEGF적표체,병탐토타문여위암림상병리특정적관계,동시용류식세포의검측S기세포비례(SPF).결과 HIF-1α재위암조직중양성표체솔위62.07％ (36/58),재암방조직중양성표체솔위18.97％(11/58),량자차이유통계학의의(x2=6.49,P＜0.05);림파결전이양성조적양성표체솔위71.88％ (23/32),림파결전이음성조적양성표체솔위26.92(7/26,x2 =6.21,P＜0.05).VEGF재위암조직중양성표체솔위65.52％(38/58),재암방조직중양성표체솔위22.41％ (13/58),량자차이유통계학의의(x2=5.32,P＜0.05);림파결전이양성조적양성표체솔위68.75％ (22/32),림파결전이음성조적양성표체솔위30.77(8/26,x2=5.43,P＜0.05).위암조직중HIF-1α화VEGF적표체여SPF치명현상관;위암조직중HIF-1 α화VEGF적표체정정상관.결론 위암조직중HIF-1α화VEGF적표체승고,차량자적표체균여림파결전이상관;HIF-1α화VEGF가이촉진위암종류세포적증식.
Objective To explore the expression of hypoxia inducible factor-1 α (HIF-1α) and vascular endothelial growth factor (VEGF) in gastric carcinoma and its relationship with tumor cell proliferation.Methods The expression of HIF-1α,and VEGF in 58 cases of gastric carcinoma tissues and 58 cases of paracancerous tissues was detected by streptavidin-peroxidase immunohistochemical staining,and S phase fraction (SPF) was assessed by flow cytometry.Results In 58 cases of gastric carcinoma tissues,the positive rate of HIF-1α was 62.07％ (36/58) and that of VEGF was 65.52％ (38/58) respectively,significantly higher than that in paracancerous tissues.The positive rate of HIF-1α and VEGF in gastric carcinoma tissues with lymph node metastasis was higher than that without lymph node metastasis [71.88％ (23/32) vs.26.92％ (7/26);x2 =6.21,P ＜0.05) and [68.75％ (22/32) vs.30.77％ (8/26);x2 =5.43,P ＜ 0.05].There was a strong association between the expression of HIF-1 α and VEGF with SPF (t =6.826;t =5.348,P ＜ 0.01).The positive expression of HIF-1 α showed a positive correlation with the positive expression of VEGF.Conclusion The raised expression of HIF-1 α and VEGF was detectable in gastric carcinoma tissues.The expression of HIF-1α and VEGF was correlated with lymph node metastasis.HIF-1 α and VEGF could make the development by promoting the proliferation of gastric carcinoma cells.
