中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
9期
2175-2178
,共4页
钱益%施文%孙晋洁%孙永强
錢益%施文%孫晉潔%孫永彊
전익%시문%손진길%손영강
结肠癌%短发夹RNA%基因沉默%趋化因子受体7
結腸癌%短髮夾RNA%基因沉默%趨化因子受體7
결장암%단발협RNA%기인침묵%추화인자수체7
Colorectal cancer%Short hairpin RNA%Small interfering%Chemokine receptor 7
目的 通过对趋化因子受体7(CXCR7)的基因干扰,观察其对人结肠癌细胞株LoVo的侵袭、转移能力的影响.方法 将靶向CXCR7基因的携带短发夹RNA(shRNA)表达的质粒转入LoVo细胞株,以体外侵袭实验观察LoVo细胞侵袭能力的改变;将36只裸鼠分成3组,行种植成瘤肝转移实验观察LoVo细胞转移能力的改变.结果 体外实验结果显示CXCR7-shRNA干扰组LoVo细胞穿膜数[(51.70 ±6.89)个]较空白对照组[(94.30±7.40)个]减少(P＜0.05);动物实验结果显示3组裸鼠的中位生存时间分别为:CXCR7-shRNA干扰组52 d、空白对照组30 d、阴性对照组32 d.CXCR7-shRNA干扰组较空白对照组生存率明显提高(P＜0.05),肝脏表面的转移瘤结节数较空白对照组明显减少(P＞0.05).空白对照组与阴性对照组比较差异无统计学意义(P＞0.05).结论 shRNA干扰技术沉默CXCR7的表达能抑制人结肠癌细胞LoVo的侵袭和转移能力.
目的 通過對趨化因子受體7(CXCR7)的基因榦擾,觀察其對人結腸癌細胞株LoVo的侵襲、轉移能力的影響.方法 將靶嚮CXCR7基因的攜帶短髮夾RNA(shRNA)錶達的質粒轉入LoVo細胞株,以體外侵襲實驗觀察LoVo細胞侵襲能力的改變;將36隻裸鼠分成3組,行種植成瘤肝轉移實驗觀察LoVo細胞轉移能力的改變.結果 體外實驗結果顯示CXCR7-shRNA榦擾組LoVo細胞穿膜數[(51.70 ±6.89)箇]較空白對照組[(94.30±7.40)箇]減少(P＜0.05);動物實驗結果顯示3組裸鼠的中位生存時間分彆為:CXCR7-shRNA榦擾組52 d、空白對照組30 d、陰性對照組32 d.CXCR7-shRNA榦擾組較空白對照組生存率明顯提高(P＜0.05),肝髒錶麵的轉移瘤結節數較空白對照組明顯減少(P＞0.05).空白對照組與陰性對照組比較差異無統計學意義(P＞0.05).結論 shRNA榦擾技術沉默CXCR7的錶達能抑製人結腸癌細胞LoVo的侵襲和轉移能力.
목적 통과대추화인자수체7(CXCR7)적기인간우,관찰기대인결장암세포주LoVo적침습、전이능력적영향.방법 장파향CXCR7기인적휴대단발협RNA(shRNA)표체적질립전입LoVo세포주,이체외침습실험관찰LoVo세포침습능력적개변;장36지라서분성3조,행충식성류간전이실험관찰LoVo세포전이능력적개변.결과 체외실험결과현시CXCR7-shRNA간우조LoVo세포천막수[(51.70 ±6.89)개]교공백대조조[(94.30±7.40)개]감소(P＜0.05);동물실험결과현시3조라서적중위생존시간분별위:CXCR7-shRNA간우조52 d、공백대조조30 d、음성대조조32 d.CXCR7-shRNA간우조교공백대조조생존솔명현제고(P＜0.05),간장표면적전이류결절수교공백대조조명현감소(P＞0.05).공백대조조여음성대조조비교차이무통계학의의(P＞0.05).결론 shRNA간우기술침묵CXCR7적표체능억제인결장암세포LoVo적침습화전이능력.
Objective To investigate the effects of chemokine receptor 7 (CXCR7) gene on the proliferation and migration of LoVo cells of human colon cancer cell line.Methods The expression vector with short hairpin RNA (shRNA) targeting CXCR7 was transfected into LoVo cells.The expression of knockdown CXCR7 gene was confirmed by Western blotting.To investigate the effects of the expression of CXCR7 on proliferation and migration of LoVo cells,Transwell migration assay was used in vitro,and tumor xenografts with liver metastasis model were performed in vivo,respectively.Results The number of LoVo cells migrating across the polycarbonate membrane in the CXCR7-shRNA transfection group (51.70 ±6.89) was significantly less than that in the control groups (94.30 ±7.40,P ＜0.05).The median survival time in CXCR7-shRNA transfection group,blank contorl group and negative control group was respectively 52,30,and 32 days.The survival rate in CXCR7-shRNA transfection group was significantly higher than in blank contorl group (P ＜ 0.05).The amount of the tumor nodules on the liver surface in nude mice of CXCR7-shRNA transfection group was dramatically reduced (P ＞ 0.05).No significant difference was found between blank contorl group and negative control group (P ＞ 0.05).Conclusion RNA interference targeting CXCR7 gene can inhibit the proliferation and migration of LoVo cells.
